Words similar to pulsed-field
Example sentences for: pulsed-field
How can you use “pulsed-field” in a sentence? Here are some example sentences to help you improve your vocabulary:
Pulsed-field gel electrophoresis was carried out in a Pharmacia/LKB apparatus under the following conditions: electrophoresis buffer, 1 × TBE (45 mM Tris-borate/1.
Each tomato BAC clone was estimated to be approximately 120 kb in length by pulsed-field gel electrophoresis (data not shown), but neither contained marker TG269 or CT190 sequences (data not shown) to allow confirmation that the dgt locus was located on either BAC clone.
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
In this work, we employed the pulsed-field gel electrophoresis procedure to measure large non-circular forms of the chromosome obtained from various recombination-defective mutants in rec +, recBC sbcA , and recBC sbcBC genetic backgrounds.
Large linear chromosomal fragments were measured by pulsed-field gel electrophoresis.
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