Words similar to pulsed-field
Example sentences for: pulsed-field
How can you use “pulsed-field” in a sentence? Here are some example sentences to help you improve your vocabulary:
Pulsed-field gel electrophoresis
To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).
Pulsed-field gel electrophoresis analysis of chromosomes after ultraviolet irradiation has revealed extensive chromosome degradation dependent on uvrA incision enzyme [ 27 ] . A report [ 28 ] showed that RecQ and RecJ proteins process nascent DNA at replication forks blocked by ultraviolet irradiation prior to the resumption of DNA synthesis (see also [ 29 ] ).
Large linear chromosomal fragments were measured by pulsed-field gel electrophoresis.
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
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