Words similar to pulsed-field
Example sentences for: pulsed-field
How can you use “pulsed-field” in a sentence? Here are some example sentences to help you improve your vocabulary:
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
When the chromosomal DNA in the agar plug was digested with a restriction enzyme ( Xba I) before the pulsed-field gel electrophoresis, all the strains examined produced comparable amounts of DNA (Figure 3).
Our operational definition of the non-circular forms is their presence in an area just below the well in our pulsed-field gel, as marked by a bar in Figure 1. The molecular species in this area may not be limited to a simple linear form of varying lengths.
Each tomato BAC clone was estimated to be approximately 120 kb in length by pulsed-field gel electrophoresis (data not shown), but neither contained marker TG269 or CT190 sequences (data not shown) to allow confirmation that the dgt locus was located on either BAC clone.
To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).
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