Words similar to pulse-labeling

Example sentences for: pulse-labeling

How can you use “pulse-labeling” in a sentence? Here are some example sentences to help you improve your vocabulary:

For pulse-labeling, the cells were washed three times with DMEM lacking Met and Cys and then incubated in the same medium containing 80 μCi/ml of 35S-labeled Met and Cys (Tran 35S-label, ICN) and the proteasome inhibitor MG-132 (10 μM, Calbiochem) for 1.5 hours.
Immunoprecipitation, pulse-labeling and CDK2 kinase assays
An important role of Chl b was further revealed by experiments in which newly synthesized LHCPs were detected by pulse-labeling in Chl b -less mutant plants but the proteins were not recovered in chloroplasts isolated from these plants [ 4 ] . These Chl b -less plants did not accumulate several of the LHCPs, in particular Lhcb1, Lhcb6 and Lhca4 [ 5 ] . Chl b was not detected in plants exposed to intermittent light (cycles of 2 min of light and 98 min of darkness), which accumulated only small amounts of Chl a and thylakoid membranes [ 6 7 ] . Wild-type plants treated in this way accumulated only one LHCP (Lhcb5), while Chl b -less mutants exposed to intermittent light lacked all LHCPs [ 8 ] . In complementary fashion, Chl b did not accumulate when synthesis of LHCPs was inhibited [ 9 ] . When bean plants exposed to intermittent light were treated with chloramphenicol to inhibit synthesis of proteins on chloroplast ribosomes, Chl