Words similar to pulse-chase

Example sentences for: pulse-chase

How can you use “pulse-chase” in a sentence? Here are some example sentences to help you improve your vocabulary:

For 35S pulse-chase labeling, cells in a 6 cm dish were washed with DMEM (without methionine), and then incubated in DMEM (without methionine) containing 10% dialyzed fetal bovine serum at 37°C for 1 h.
Co-expression of Rlk and Fyn also led to a rapid disappearance of Rlk from the detergent soluble fraction as measured by pulse-chase analysis (Fig.
A pulse-chase protocol was also used to study post-transcriptional regulation of APP metabolsm by statins (data not shown).
Lastly, 35S-methionine pulse-chase labeling studies directly confirmed that sα i2 was more rapidly degraded than α i2 (Figure 10).
The following results support this hypothesis: 1) sα i2 and α i2 are equally labeled by a pulse of 3H-myristate, although much less sα i2 protein is detected; 2) sα i2 displays a propensity to localize to potential aggresome-like structures, and this localization is greatly enhanced by proteasome inhibitor treatment; 3) the α i2 A327S mutant, previously shown to be unstable and defective in guanine-nucleotide binding, shows a similar pattern of subcellular localization ( i.e., intracellular membranes rather than PM); 4) βγ over-expression increases expression of sα i2 and promotes PM localization of sα i2 and α i2 A327S, but βγ co-expression does not prevent sα i2 localization to potential aggresome-like structures when cells are treated with proteasome inhibitors; and 5) pulse-chase analysis indicates that sα i2 is rapidly degraded.