Example sentences for: psv

How can you use “psv” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For the transfection control, pSV110 was transfected into Vero cells, mock-infected and directly transformed into bacteria for blue/white colony screening.

  • These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.

  • Since the process of transfection is often mutagenic, pSV110 was transfected into Vero cells, mock-infected and rescued into bacteria to determine background frequency of mutation from the experimental process.

  • A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (ori S ) SmaI fragment [ 5 ] . The plasmid pTK1 contains HSV-1 SC16 sequence coordinates 45,055-48,634 subcloned into the HindIII / EcoRI site of pUC19 (Invitrogen).

  • The following day, cells were transfected with 1.5 μg of pSV110 and 1 μg of salmon sperm DNA using Lipofectamine reagent according to the manufacturer's recommendations (Invitrogen).


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