Words similar to psv
Example sentences for: psv
How can you use “psv” in a sentence? Here are some example sentences to help you improve your vocabulary:
A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (ori S ) SmaI fragment [ 5 ] . The plasmid pTK1 contains HSV-1 SC16 sequence coordinates 45,055-48,634 subcloned into the HindIII / EcoRI site of pUC19 (Invitrogen).
Since the process of transfection is often mutagenic, pSV110 was transfected into Vero cells, mock-infected and rescued into bacteria to determine background frequency of mutation from the experimental process.
The following day, cells were transfected with 1.5 μg of pSV110 and 1 μg of salmon sperm DNA using Lipofectamine reagent according to the manufacturer's recommendations (Invitrogen).
For the transfection control, pSV110 was transfected into Vero cells, mock-infected and directly transformed into bacteria for blue/white colony screening.
For the superinfection control, pSV110 was transfected into Vero cells, superinfected with HP66 Pol-mutant virus (which is unable to replicate) and transformed into bacteria for blue/white colony screening.