Words similar to psp
Example sentences for: psp
How can you use “psp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Development of our control set of 10 Arabidopsis oligonucleotides and corresponding set of PCR amplicons subcloned into pSP64poly(A) serve as a valuable quality-control resource for cDNA/oligo microarrays.
To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).
For printing Arabidopsis genes, inserts in pSP64poly(A) were amplified by PCR with the SP6 (5'-ATTTAGGTGACACTATAG-3') and M13R primers.
PCR products were digested with the restriction enzymes Hind III and Sac I, subcloned into pSP64 poly(A) vector (Promega, Madison, WI) and sequence verified.
The coding region for Xenopus Wnt-5a including the myc epitope tag in pSP64T [ 45 ] was digested with BglII, treated with klenow enzyme and ligated into the retroviral vector PMI-hCD5 [ 46 ] digested with NotI and treated with Klenow enzyme.