Example sentences for: psp

How can you use “psp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For printing Arabidopsis genes, inserts in pSP64poly(A) were amplified by PCR with the SP6 (5'-ATTTAGGTGACACTATAG-3') and M13R primers.

  • PCR products were digested with the restriction enzymes Hind III and Sac I, subcloned into pSP64 poly(A) vector (Promega, Madison, WI) and sequence verified.

  • Development of our control set of 10 Arabidopsis oligonucleotides and corresponding set of PCR amplicons subcloned into pSP64poly(A) serve as a valuable quality-control resource for cDNA/oligo microarrays.

  • The coding region for Xenopus Wnt-5a including the myc epitope tag in pSP64T [ 45 ] was digested with BglII, treated with klenow enzyme and ligated into the retroviral vector PMI-hCD5 [ 46 ] digested with NotI and treated with Klenow enzyme.

  • To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).


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