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Example sentences for: psw

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Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.
The PCR product was digested with SacI and XbaI and cloned into the SacI/XbaI sites of both pRS315 (pSW1264) and pRS316 (pSW1268) [ 66 ] . Plasmids with wild type SEC23 were constructed in the same manner using specific oligonucleotides (5' oligo sec23F5 with 5'-ATGAATATCTAGACCAGGGTGCC-3'; 3' oligo sec23-3 with 5'CCTTGGATCCGTAGTAAAGGCCACGCAG-3') and digestion of the PCR product with XbaI and BamHI to yield p SEC23/LEU2 (pSW1266) and p SEC23/URA3 (pSW1269).
A partial deletion of SEC27 from one of the inserts (pSW1386) was used to confirm that SEC27 was responsible for rescue of the growth defect.
To test the function of the isolated mutant alleles, the resulting haploid secΔ pSEC/URA3/CEN strains were transformed with the mutant sec alleles in a LEU2/CEN plasmid (pSW1264 or pSW1266; see below).