Words similar to promoterless

Example sentences for: promoterless

How can you use “promoterless” in a sentence? Here are some example sentences to help you improve your vocabulary:

1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.
4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
The plasmid contains a unique Bam HI site upstream of the promoterless reporter operon for cloning of potential promoters.
2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the Eco RI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).
The basic I-TRAP approach (Figure 1) uses a genomic DNA library in a plasmid vector containing a promoterless 'operon' with two reporter genes.