Example sentences for: promoterless

How can you use “promoterless” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The basic I-TRAP approach (Figure 1) uses a genomic DNA library in a plasmid vector containing a promoterless 'operon' with two reporter genes.

  • 2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the Eco RI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.

  • The plasmid contains a unique Bam HI site upstream of the promoterless reporter operon for cloning of potential promoters.

  • The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).


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