Words similar to promoter-trap
Example sentences for: promoter-trap
How can you use “promoter-trap” in a sentence? Here are some example sentences to help you improve your vocabulary:
Construction of a library in the promoter-trap plasmid
To develop the I-TRAP method for identifying M. tuberculosis promoters dependent on the expression of σ E, a strain of M. smegmatis over-expressing the M. tuberculosis σ E, a σ Emutant strain of M. smegmatis, and a M. tuberculosis promoter-trap library were created.
To identify transcription factor-dependent promoters, a genomic DNA library is generated in a promoter-trap plasmid containing two reporter genes.
4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the Eco RI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).