Words similar to probed

Example sentences for: probed

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Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).
5 mM Tris, pH 6.7) at 50°C for 30 min, washed 4 times with TBS-Tween, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-p44/42 ERK antibody according to the manufacturer's recommendations.
Similarly to those probed with the ATPase 6 gene, cells in the apical area of alveoli (2-3 per alveolus) and epithelial cells lining the distal airways of NRL sections showed blue precipitate when reacted with the anti-sense SP-B probe (Fig.
The band intensities of the same blot probed with a fragment of the 18S rRNA gene were used as an internal control for variations in the amount of RNA loaded on the blot (Fig.
Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.