Words similar to preincubation

Example sentences for: preincubation

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TGFβ stimulation of cells (5 ng/ml) was performed 15 hours after transfection for the indicated times with or without preincubation with the respective inhibitors.
Preincubation with both antibodies results in further retardation of the binding complex to form a doublet (Fig.
This preincubation also decreased activation of p21Ras and of MAPK ERK1/2, indicating the participation of the Ras/MAPK-pathway in TGFβ 1 induced transcriptional activation.
Pituitary cells that had been re-trypsinized 72 h after infection and then replated (100,000 cells/well in a 24-well poly-L-lysine coated plate) for a 2 h preincubation were subsequently treated with a GnRH agonist (1 ml of 10 -7M D-Ala 6- des Gly 10- GnRH ethylamide; GnRH-A, Sigma, St. Louis, MO) or a calcium ionophore (A23187, Sigma) for 3 h as described by Conn et al.
After preincubation with protein A-sepharose 4CL, the cell extract was incubated with specific mAb directly conjugated to sepharose 4B for 60 min, washed 3 times with the appropriate lysis buffer and eluted with Laemmli sample buffer.