Words similar to preincubation
Example sentences for: preincubation
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Moreover, the reaction with BMP-5 protein is significantly blocked by preincubation of the BMP-5 Ab with blocking peptide (Figure 6).
After a further 24 h, the pituitary cells were recovered by trypinization, counted, and replated in balanced salt solution (400,000 - 500,000 cells/well in a 24-well plate) for a 2 h preincubation.
The same attenuation of TGFβ 1 induced gene expression and the decrease in p21Ras and MAPK ERK1/2 activation was observable after preincubation of SMAD4 wildtype cells with α V β 6 -blocking antibodies, demonstrating that TGFβ 1 signaling via α V β 6 -integrin also is linked to the Ras/MAPK-pathway, and that both pathways have synergistic effects in TGFβ 1 -signaling.
The same electrophoretic patterns were seen after preincubation of nuclear extracts from the CEM T lymphocytoid cell line with anti-DEK and anti-NF-YA antibodies (data not shown).
Third, in rat spinal cord ( 12), we found that preincubation of fixed tissue with specific δ-opioid ligands (DADLE, Met-enkephalin, dermenkephalin and naltrindole at 10 -9M) totally prevented the antibody labeling, showing a good competition between ligands and the anti-Id mAb.