Words similar to preincubation

Example sentences for: preincubation

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We prepared nuclear and whole cell extracts from cells treated with TGFβ with or without preincubation of one of the SB inhibitors and analyzed those extracts for reactivity to a Smad2,3-specific antibody by Western blot analysis.
Moreover, control sections were incubated in the primary antiserum previously absorbed by neurokinin A or neurokinin B. Inhibition of staining was obtained after preincubation of the first antiserum with neurokinin A (10 -4and 10 -5M) or with neurokinin B (10 -3M).
This TGFβ 1 -induced expression profile was not affected by preincubation of SMAD4 deleted BxPC-3 cells with a TGFβ 1 -RII blocking antibody, which was able to completely block TGFβ 1 -induced SMAD2/3 phosphorylation, thus demonstrating the independence of the TGFβ 1 -signaling from the known SMAD pathway in BxPC-3 cells.
This preincubation also decreased activation of p21Ras and of MAPK ERK1/2, indicating the participation of the Ras/MAPK-pathway in TGFβ 1 induced transcriptional activation.
Remarkably, preincubation with the MEK1 inhibitor PD98059 resulted in a reduced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . This finding is in agreement with other observations that MEK1-mediated signal transduction is involved in cytoskeletal remodeling and integrin engagement [ 70 71 ] .