Words similar to prehybridization
Example sentences for: prehybridization
How can you use “prehybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:
The DNA on the microarray was then subjected to a UV-cross-linking at a dose of 150 mJ/cm 2. Prehybridization was performed by incubating the slide in a fresh mixture of 100 ml of 25% formamide, 5X SSC, 0.1% SDS, and 1% BSA for 45 min. at 42°C in a Coplin jar.
Prehybridization was performed at 67°C for two hours in a seal-a-meal bag (Dazey Corporation, Industrial Airport, KS) with hybridization solution (0.
Just prior to prewetting in wash buffer for prehybridization RNA membranes were given two deglyoxylation washes in 20 mM Tris pH 8 for twenty minutes each at 65°C.
Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.
Prehybridization and hybridization were performed at 42°C and using Quick Hyb ®(Stratagene) as hybridization solution.