Words similar to prehybridization
Example sentences for: prehybridization
How can you use “prehybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:
Prehybridization was performed at 67°C for two hours in a seal-a-meal bag (Dazey Corporation, Industrial Airport, KS) with hybridization solution (0.
Just prior to prewetting in wash buffer for prehybridization RNA membranes were given two deglyoxylation washes in 20 mM Tris pH 8 for twenty minutes each at 65°C.
The hybridization solution was the same as the prehybridization solution but contained 10 6cpm/ml of the cCAF probe or with the same number of counts of the vector probe and incubated at 55°C for 48 h with gentle shaking.
Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.
Prehybridization and hybridization were performed at 42°C and using Quick Hyb ®(Stratagene) as hybridization solution.
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