Words similar to prehybridization
Example sentences for: prehybridization
How can you use “prehybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:
Prehybridization and hybridization steps were carried out at 65°C in Rapid-Hyb solution according to manufacturer's instructions.
The DNA on the microarray was then subjected to a UV-cross-linking at a dose of 150 mJ/cm 2. Prehybridization was performed by incubating the slide in a fresh mixture of 100 ml of 25% formamide, 5X SSC, 0.1% SDS, and 1% BSA for 45 min. at 42°C in a Coplin jar.
The hybridization solution was the same as the prehybridization solution but contained 10 6cpm/ml of the cCAF probe or with the same number of counts of the vector probe and incubated at 55°C for 48 h with gentle shaking.
The probe was denatured by heating to 95°C for 3 min before adding to the prehybridization solution.
Prehybridization and hybridization were performed at 42°C and using Quick Hyb ®(Stratagene) as hybridization solution.