Example sentences for: prehybridized

How can you use “prehybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • The membranes were prehybridized in a commercial Ultrahyb buffer (Ambion, Inc., Austin, TX) for 1 to 2 hours at 65°C.

  • Blots were prehybridized and hybridized at 42°C in ExpressHyb (Clontech, Palo Alto, CA) and washed in 0.1× SSC/1% SDS for one hour at 65°C.

  • Slides were prehybridized in 1.0% BSA, 5 × SSC, 0.1% SDS for 45 min, washed by repeated dipping in MilliQ water twice and 2-propanol once, and air dried.


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