Example sentences for: prehybridized

How can you use “prehybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A Research Genetics gene filter (GF201) was prehybridized in MicroHyb solution (Research Genetics) containing 5 μg Cot-1 DNA (Life Technologies) and 5 μg poly dA at 42°C for at least 2.5 hr.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • The membrane was prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C with random primer-labeled lac operator concatamer (40 copies) probe in ExpressHyb hybridization solution (Clontech Laboratories, CA), and then washed at 60°C in washing solution (0.

  • The membranes were prehybridized in a commercial Ultrahyb buffer (Ambion, Inc., Austin, TX) for 1 to 2 hours at 65°C.

  • The filters were prehybridized for 1.5 h, hybridized overnight at 62.


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