Example sentences for: prehybridized

How can you use “prehybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Slides were prehybridized in 1.0% BSA, 5 × SSC, 0.1% SDS for 45 min, washed by repeated dipping in MilliQ water twice and 2-propanol once, and air dried.

  • Isolation of total cellular RNA using a urea/LiCl protocol and gel electrophoresis was performed as described previously [ 65 ] . Gels were transferred onto Amersham Hybond N+ nylon membranes in 20 × SSC and prehybridized in 4 × SSC, 1 × Denhardt's solution, 0.5% SDS for 2 h at 68°C.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • A Research Genetics gene filter (GF201) was prehybridized in MicroHyb solution (Research Genetics) containing 5 μg Cot-1 DNA (Life Technologies) and 5 μg poly dA at 42°C for at least 2.5 hr.

  • The second was the random primer-labeled LacI/NLS . Membranes were prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C in ExpressHyb hybridization solution and washed at 60°C in washing solution.


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