Example sentences for: prehybridized

How can you use “prehybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A Research Genetics gene filter (GF201) was prehybridized in MicroHyb solution (Research Genetics) containing 5 μg Cot-1 DNA (Life Technologies) and 5 μg poly dA at 42°C for at least 2.5 hr.

  • The membranes were prehybridized in a commercial Ultrahyb buffer (Ambion, Inc., Austin, TX) for 1 to 2 hours at 65°C.

  • Blots were then prehybridized overnight at 42°C in 50% formamide, 4X SSC, 5X Denhart's solution, 25 mM sodium phosphate, pH 6.5, 1.0% sodium deodecyi sulphate (SDS), and 200 μg/ml salmon sperm DNA.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • Room-temperature probes were applied to a prehybridized array, covered with a glass coverslip (Fisher), and placed in a humidified hybridization chamber (Corning).


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