Example sentences for: prehybridized

How can you use “prehybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sections were prehybridized for 2 h at 54°C in 500 μl hybridization buffer (50% formamide, 5x SSC, 40 μg/ml salmon sperm DNA) before hybridization with heat denatured RNA probes which had been diluted to 400 ng/ml in hybridization buffer.

  • The second was the random primer-labeled LacI/NLS . Membranes were prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C in ExpressHyb hybridization solution and washed at 60°C in washing solution.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • Membranes were prehybridized for 2 hrs in Ambion Hybridization buffer at 62°C, and hybridized O/N at 62°C.

  • The membranes were prehybridized in a commercial Ultrahyb buffer (Ambion, Inc., Austin, TX) for 1 to 2 hours at 65°C.


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