Words similar to preincubated
Example sentences for: preincubated
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To quantitate differences in activated (phosphorylated) p53 protein, 3 SN50 and control E15 + 2 explants were sectioned, preincubated with unlabeled goat-anti mouse IgG as previously described [ 9 ] and sequentially incubated with a monoclonal anti-phosphorylated p53 (Ser15) antibody (Cell Signaling Technology, Beverly, MA), biotin-labeled goat anti-mouse IgG, and HRP-labeled SA (Zymed Laboratories, South San Francisco, CA), and counterstained with hematoxylin.
In part the cells were preincubated with α
AGE-BSA5-induced apoptosis was blocked when cells were preincubated with neutralizing anti-CD36 antibody, while preincubation with control IgG antibody had no effect (Figure 4A).
The procedure for the histochemical demonstration of actomyosin ATPase was employed as described by Goth and Samara [ 24 ] . Briefly, fixed sections were washed in rinse solution [18 mM calcium chloride in 100 mM tris (hydroxymethyl) aminomethane (Tris), pH 7.8] for 1 minute, preincubated either in 50 mM potassium acetate in 18 mM calcium chloride at pH 4.35 (for acid-stable myosin ATPase, type I slow twitch muscle fibers), or in 18 mM calcium chloride in 100 mM 2-amino-2-methyl-1-propanol at pH 10.
To examine the effects of increasing doses of CDDO in human primary chondrocytes, cells were preincubated for 24 hours with CDDO (300 nM to 5 μM), or with medium alone, before the addition of IL-1 for 18 hours.