Words similar to preincubated

Example sentences for: preincubated

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In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .
In the use of BIM, islets or cells were preincubated in the solution containing BIM (50 μM) for 30-60 min, and BIM was present in the bath solution throughout the experiment
Each section was preincubated with 2% bovine serum albumin for 1 hour, then incubated overnight at 4°C with the appropriate primary antibody at 1:500 dilution.
Prior to CSC challenge, the transfected cells were preincubated for 4 hours in minimal Eagle's medium with Earle's salts to remove serum factors.
In part the cells were preincubated with α V - and β 6 -antibodies (1:100 each for 30 min), with a TGFβ-RII antibody (15 μg/ml for 30 min), cytochalasin D, BAPTA AM and MEK1 inhibitor PD98059, respectively.