Example sentences for: polymerase

How can you use “polymerase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR reaction mix consisted of 20 mM Tris-HCl (pH 8.4), 50 mM KCl, 0.25 mM each of dNTPs, 2.5 mM MgCl 2 , 0.2 μM each primer, and 0.6 units Tsp polymerase (Platinum GenoTYPE Tsp DNA Polymerase, Cat # 11448-032, Invitrogen, Carlsbad, CA).

  • We speculated that alanine substitution or deletion in the loop of the p51 subunit may shorten the interacting sphere of the loop which may not be able to induce opening of the polymerase cleft in p66, which is essential for DNA binding.

  • These obstacles have been overcome by two related protocols, both of which are based on the polymerase chain reaction (PCR).

  • Standard RNA was made for all assays by the T7 polymerase method (Ambion, Austin, TX), using total RNA isolated from the mouse heart.

  • Samples underwent 30 cycles of amplification with Perkin-Elmer Taq polymerase in a PTC Thermal Cycler (MJ Research, MA) (94° for 40 sec, 57° for 1 min, 72° for 2 min).


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