Example sentences for: polymerase

How can you use “polymerase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Taq polymerase was purchased from Promega (Madison, WI), and Oligo dT and ficoll/isopaque were purchased from Pharmacia (Piscataway, NJ).

  • A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was developed for genotyping.

  • The reactions contained 100 ng template DNA, 40 pmoles of primer, 2.5 mM Mg 2+ions, 200 μM each dNTP, 0.3 U Taq DNA polymerase in 1× assay buffer in a final volume of 25 μl.

  • Because the Taq and Stoffel polymerase both reached the same optimum S/N of 100, it seems most likely that template damage via heating-cooling cycles as found by Gustafson et al.

  • Amplification reactions consisted of a 25 μl reaction containing 100 ng genomic DNA or 20 ng BAC DNA, 200 μM dNTPs, 200 nM each forward and reverse primer, 1 × reaction buffer (10 mM Tris-HCl pH 8.3, 50 mM KCl), 1.5 mM MgCl 2 , and 1 U Maxi Taq polymerase [ 36].


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