Example sentences for: polymerase

How can you use “polymerase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In general, PCR reactions were performed in a volume of 50 μl with 50 picomoles of each primer, dNTP's (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) at a final concentration of 0.5 mM, 1 μl of template, 1X PCR buffer (10X PCR buffer contained 700 mM Tris pH 8.8, 20 mM MgCl 2 , 1% Triton X-100, and 0.1% Tween 20) and Taq polymerase (Perkin-Elmer Inc., Wellesley MA) diluted to the manufacturers recommendations.

  • This combination of residues has not previously been shown to yield an active T7 RNA polymerase promoter.

  • For example, a PSI-BLAST search initiated with the SBHM from NlpD retrieved the derived SBHMs of the bacterial RNA polymerase subunits (e.g.

  • PCR was "hot start", i.e., the polymerase mixed in 10% of the final reaction volume in 1 × buffer was added to the rest of the mix during the initial denaturing step when the sample temperature was already at 94°C.

  • These conditions were chosen because the RNAseH and polymerase activities function coordinately during reverse transcription, and hence it is reasonable to assume they operate under similar conditions.


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