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Example sentences for: polymerases

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Template-independent RNA polymerases (nucleotidyltransferases) include CCA-adding enzymes, polyA polymerases, uridylyl transferases and oligoA-synthetases.
Closely spaced acidic residues that coordinate divalent cations are characteristic of the active sites of most nucleic acid polymerases, in spite of the fact that they belong to several unrelated structural folds [ 9 13 14 17 28 29 59 ] . Given that the DbDGD motif is the only set of closely spaced acidic residues shared by the RDRPs and the YRHs, it is likely to form part of the nucleotidyltransferase active site of these enzymes.
For example, the only known polymerase variant that switches promoters contains a single amino acid change, recognizes a single nucleotide change in the promoter, and closely mimics interactions known to occur for T3 RNA polymerase [ 9 ] . The large sequence space that surrounds both polymerases and promoters has so far prevented more sweeping searches for more diverse variants.
The pol from HSV-2 6757, a virus which showed a 300- to 700-fold higher error rate than HSV-1 SC16 by the tk mutagenesis assay previously [ 21 ] , was found to contain three mutations compared to the other HSV-2 pols , with one change residing in region VII which is shared among diverse DNA polymerases (Fig.
First, two different thermostable polymerases ( Taq and Pwo polymerase) were used to generate the primary amplification products for cloning and subsequent sequence analysis.