Words similar to polony
Example sentences for: polony
How can you use “polony” in a sentence? Here are some example sentences to help you improve your vocabulary:
Adherence to mutational hotspots was directly responsible for the inability to detect the p53 mutations in the CAPAN and AsPc-1 cell lines [ 19 21 ] . The best approach for detecting mutations outside the hotspots will involve direct sequencing of the polony PCR products [ 2 13 14 ] .
Previous work has shown that a significant percentage of mutations in p53 and K- ras 2 are localized to mutational hotspots, namely codons 175, 245, 248, 249, 273, and 282 in p53 and codons 12, 13, and 61 in K- ras 2 [reviewed in [ 14 17 18 ] ]. Each of these mutational hotspots was sequenced in the genomic DNA of various pancreatic cell lines using polony technology as follows.
Denaturation and electrophoresis of polony gels
Herein, we describe the applications of polony technology to cancer research with the goal of screening for: 1) mutations in key tumor suppressor and oncogenes; as well as 2) loss of heterozygosity.
It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).