Words similar to polony

Example sentences for: polony

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For example, previous studies have shown that Panc-1 has two copies of K- ras 2, but only one copy of p53, due to LOH [ 20 ] . When equivalent quantities of Panc-1 genomic DNA were polony amplified, there were approximately twice as many K- ras 2 polonies as there were p53 polonies (Figure 3).
Initially, each exon bearing a mutational hotspot was individually PCR amplified in a polyacrylamide gel giving rise to one polymerase colony, or polony, per copy of genomic p53 or K- ras 2 DNA.
Of particular importance to this study are the genes p53 and K- ras 2 since a significant proportion of the mutations are localized to a relatively small number of mutational hotspots [ 13 14 15 16 ] . In this paper we introduce a screen to detect the presence of pancreatic cancer by looking for somatic mutations in p53 and K- ras 2 using polony technology to microsequence mutational hotspots within these two genes.
For each position within a mutational hotspot to be genotyped, 20 μL of master mix was combined with 1 μL of genomic DNA as well as 0.23 μL of each the forward and reverse primers (50 μM each; see Table 1) designed to polony amplify the portion of the exon bearing the mutational hotspot(s).
Polony technology provides this capability due to its "digital" nature, where one DNA molecule gives rise to one polony.