Words similar to polony

Example sentences for: polony

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Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
The non-acrydited strand of the polony was then stripped away after formamide treatment and electrophoresis.
Of particular importance to this study are the genes p53 and K- ras 2 since a significant proportion of the mutations are localized to a relatively small number of mutational hotspots [ 13 14 15 16 ] . In this paper we introduce a screen to detect the presence of pancreatic cancer by looking for somatic mutations in p53 and K- ras 2 using polony technology to microsequence mutational hotspots within these two genes.
Previous work has shown that a significant percentage of mutations in p53 and K- ras 2 are localized to mutational hotspots, namely codons 175, 245, 248, 249, 273, and 282 in p53 and codons 12, 13, and 61 in K- ras 2 [reviewed in [ 14 17 18 ] ]. Each of these mutational hotspots was sequenced in the genomic DNA of various pancreatic cell lines using polony technology as follows.
After electrophoresis, the polony slides were washed 4× in wash 1E (0.