Example sentences for: polony

How can you use “polony” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Adherence to mutational hotspots was directly responsible for the inability to detect the p53 mutations in the CAPAN and AsPc-1 cell lines [ 19 21 ] . The best approach for detecting mutations outside the hotspots will involve direct sequencing of the polony PCR products [ 2 13 14 ] .

  • Previous work has shown that a significant percentage of mutations in p53 and K- ras 2 are localized to mutational hotspots, namely codons 175, 245, 248, 249, 273, and 282 in p53 and codons 12, 13, and 61 in K- ras 2 [reviewed in [ 14 17 18 ] ]. Each of these mutational hotspots was sequenced in the genomic DNA of various pancreatic cell lines using polony technology as follows.

  • Denaturation and electrophoresis of polony gels

  • Herein, we describe the applications of polony technology to cancer research with the goal of screening for: 1) mutations in key tumor suppressor and oncogenes; as well as 2) loss of heterozygosity.

  • It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).


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