Example sentences for: polonies

How can you use “polonies” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For example, previous studies have shown that Panc-1 has two copies of K- ras 2, but only one copy of p53, due to LOH [ 20 ] . When equivalent quantities of Panc-1 genomic DNA were polony amplified, there were approximately twice as many K- ras 2 polonies as there were p53 polonies (Figure 3).

  • Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.

  • As a result, polonies can be used to detect changes in gene copy number that arise due to either deletions or multiplications.

  • It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).

  • In addition polonies are used to diagnose LOH in these same genes.


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