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Example sentences for: polonies

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One hallmark of cancer that can be readily observed using polonies is loss of heterozygosity (LOH).
It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).
These results, taken in conjunction with the in situ genotyping shown previously in Figures 2and 3, clearly demonstrate the ability of polonies to detect LOH.
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
For example, previous studies have shown that Panc-1 has two copies of K- ras 2, but only one copy of p53, due to LOH [ 20 ] . When equivalent quantities of Panc-1 genomic DNA were polony amplified, there were approximately twice as many K- ras 2 polonies as there were p53 polonies (Figure 3).