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Example sentences for: polonies

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For example, previous studies have shown that Panc-1 has two copies of K- ras 2, but only one copy of p53, due to LOH [ 20 ] . When equivalent quantities of Panc-1 genomic DNA were polony amplified, there were approximately twice as many K- ras 2 polonies as there were p53 polonies (Figure 3).
One hallmark of cancer that can be readily observed using polonies is loss of heterozygosity (LOH).
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
This eliminates the role of primer bias contributing to the distinct number of p53 and K- ras 2 polonies amplified in Panc-1 genomic DNA.
However, polonies seem well suited for this type of analysis since several hundred to several million [ 2 ] polonies for a particular gene can be analyzed on a single slide, thereby increasing the likelihood of detecting a rare event [ 22 ] . Additionally, the frequency of mutations can be readily determined using this approach due to the "digital" nature of polonies.