Words similar to plc
Example sentences for: plc
How can you use “plc” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although these results suggest that blockade of MTX-induced responses by the U-compounds is independent of PLC, they identify these compounds as novel, potent, and rapid blockers of MTX action.
It is however, well established that MTX causes the hydrolysis of phosphoinositides in some cell types, presumably via activation of phospholipase C (PLC) [ 12 13 ] . Activation of PLC by MTX appears to be indirect resulting as a consequence of increased [Ca 2+] i . These results suggest that PLC may be involved in activation of COP and/or in the cytolysis phase of MTX action.
To test the hypothesis that MTX-induced cell death requires PLC, the effect of U73122, a specific inhibitor of PLC was examined.
However, both compounds have been shown to produce non-specific effects presumably unrelated to inhibition of PLC [ 15 16 17 18 19 20 ] . The results of the present study show, that both U73122 and U73343 inhibit MTX-induced change in [Ca 2+] i , ethidium uptake, and LDH release in BAECs.
Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
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