Words similar to pkl

Example sentences for: pkl

How can you use “pkl” in a sentence? Here are some example sentences to help you improve your vocabulary:

In pKL106, the wild type λ repressor is tagged with the myc epitope and the LA57 λ repressor has the FLAG epitope.
In pKL107, the wild type λ repressor contains the FLAG eiptope and the LA57 mutant has the myc epitope.
All bacterial strains used were derivatives of E. coli K12 strain MC4100 [ 29 ] . IM104 is MC4100 lamB14D lon ::Tn 10 degP :: kan and IM105 is IM104 prlA4 . Plasmids pKL106 and pKL107 are pET11c (Novagen) derivatives carrying tandem copies of the λ repressor fragment (derived from plasmids pAD103 and pAD105, generous gifts from Alan Davidson, University of Toronto and Robert Sauer, MIT).
In all experiments, only antibodies to the FLAG epitope were used, therefore pKL106 resulted in detection of the mutant λ repressor and pKL107 was used to observe the wild type.
Plasmids pKL106 and pKL107 encoding LA57-N 102LT-FLAG or N 102LT-FLAG, respectively, were transformed into strains IM104 (wild type secY ) or IM105 ( prlA4 ). These strains contained deletion mutations in the lon and degP genes to limit proteolysis in both the cytoplasmic and periplasmic spaces.