Example sentences for: pkk-rt

How can you use “pkk-rt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A unique Sac I site was also introduced in pKK-RT66 template at codon 440.

  • The construction of P51+6aa was carried out by removal of the 360 bp fragment from pKK-RT66+6aa by restriction digestion with SacI followed by re-ligation of the vector ends.

  • The 18-mer duplex DNA encoding the insertion peptide (Ile-Asn-Asn-Glu-Thr-Ile) was ligated with Hpa1 digested pKK-RT66 in between codon 135 and 136.

  • The pKK-RT66 clone containing two unique restriction sites, Hpa1 and Stu1 , at codons 136 and 140 in the RT coding region [ 15 ] was used for insertion of 6 amino-acid residues in the β7-β8 loop of the p66 and p51 subunit.

  • The pKK-RT66 clone was digested with HpaI restriction enzyme to generate a blunt end at codon 136.

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