Words similar to phyto-free
Example sentences for: phyto-free
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Phyto-free males) and the greater affinity of phytoestrogens is for ER-β > ER-α, it has been shown that estrogen-related neuronal apoptosis is determined by ER-β and the Fas/Fas ligand system [ 57 ] . In fact isoflavones have been shown to cause apoptosis in rat primary cortical neurons in vitro via a calcium dependant mechanism [ 58 ] . Furthermore, the action of phytoestrogens may be tissue site specific.
Brain phytoestrogen levels are displayed in Table 1. In general, in all brain regions examined (except the hippocampus) Phyto-600 fed males displayed significantly higher phytoestrogen levels compared to Phyto-free fed males.
For the Phyto-free diet, the phytoestrogen concentrations were below the detectable limits of HPLC analysis [ 40 ] . The content and nutrient composition of these diets are described in detail elsewhere [ 40 ] . At 80-85 days of age, the animals were time mated within their respective diet treatments so that the offspring of these pairings would be exposed solely to either the Phyto-600 or Phyto-free diet.
Upon arrival all animals were allowed ad libitum access to either a commercially available diet with high phytoestrogen levels (Harlan Teklad Rodent Diet 8604, Madison, WI, USA) containing 600 micrograms of phytoestrogens/gram of diet; referred to hereafter as the Phyto-600 diet, or a custom phytoestrogen-free diet; referred to hereafter as the Phyto-free diet, obtained from Ziegler Bros. (Gardner, PA, USA) and water.
Further analysis via post hoc comparisons revealed that Phyto-600 females made significantly more correct arm choices than females switched to the Phyto-free diet during trial 10-12 ( p < 0.05) and Phyto-600 males (on trials 10-12 and 13-15; p < 0.05).
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