Example sentences for: phenol

How can you use “phenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For soft agar assay, clones were plated into 10 60-mm dishes at 1 × 10 5cells/dish containing 0.3% bactopeptone agar with or without added estrogen (10 -8mol/l) in phenol red-free medium with 10% stripped serum in order to test for anchorage independent growth.

  • 2 M sodium hydroxide and 1.0% (w/v) sodium dodecyl sulfate (SDS)), phenol and neutralization solution (3 M sodium acetate (pH5.

  • Plasmids were retested by isolation from yeast using the phenol/glass bead method followed by electroporation into bacteria [ 32 ] . The plasmids were then isolated from bacteria using a commercial DNA miniprep kit (Qiagen) and re-transformed in to yVT87.

  • HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.

  • The phenol extraction and ethanol precipitation after end repair and ligation steps were replaced with purification using Qiagen PCR Cleanup columns.


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