Example sentences for: phenol

How can you use “phenol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The phenol extraction and ethanol precipitation after end repair and ligation steps were replaced with purification using Qiagen PCR Cleanup columns.

  • Clones were isolated from the BRCA1 antisense and LXSN BG-1 pooled populations and grown in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with charcoal/dextran treated serum (Hyclone) for 5 days before plating for growth curve analysis.

  • We increased the amount of mRNA to 3-4 μg from the 2 μg recommended by the manufacturer to compensate for the loss of mRNA during the phenol chloroform extractions.

  • HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.

  • Total RNA was prepared from W303 and WHT4-1 yeast cells using hot acid phenol extraction [ 41 ] . For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.


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