Words similar to phenol
Example sentences for: phenol
How can you use “phenol” in a sentence? Here are some example sentences to help you improve your vocabulary:
The phenol extraction and ethanol precipitation after end repair and ligation steps were replaced with purification using Qiagen PCR Cleanup columns.
Clones were isolated from the BRCA1 antisense and LXSN BG-1 pooled populations and grown in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with charcoal/dextran treated serum (Hyclone) for 5 days before plating for growth curve analysis.
We increased the amount of mRNA to 3-4 μg from the 2 μg recommended by the manufacturer to compensate for the loss of mRNA during the phenol chloroform extractions.
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
Total RNA was prepared from W303 and WHT4-1 yeast cells using hot acid phenol extraction [ 41 ] . For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.
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