Words similar to phenol-red-free

Example sentences for: phenol-red-free

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For determination of basal gene-expression profiles without androgen stimulation, fibroblasts were cultured on 150-mm plastic dishes at 37°C with 5% CO 2 . To eliminate possible artifacts due to differing states of proliferation, cells were grown to confluence, at which point they enter G 0 arrest [ 31 ] . They were maintained in phenol-red-free DMEM F12 (Dulbecco's modified Eagle Medium with the nutrient mix F12; Gibco) containing L-glutamine, 15 mM Hepes buffer, penicillin/streptomycin (Gibco) and 12.
Androgen stimulation of genital fibroblasts was carried out under two different culture conditions similar to those previously reported to produce induction of aromatase enzymatic activity in these cell lines [ 8 27 ] . In the first, cells were grown to confluence as described above using phenol-red-free DMEM F12 containing L-glutamine, 15 mM Hepes buffer, penicillin/streptomycin (Gibco) with 12.