Words similar to phe-

Example sentences for: phe-

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Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
Alexa 488 and BODIPY TR were used to label dermorphin, deltrophin, TIPP (Try-Tic-Phe-Phe) and endomorphin by adding a cysteine to each peptide at the C-terminus, followed by conjugation of the fluorophores to the cysteine residue.
Following incubation for 30 minutes at 37°C 150 μL of the substrate buffer solution, Bz-Pro-Phe-Arg-paranitroanilide, (Sigma Chemical) was added to each well.
After two rounds of routing, synthesis, and selection, followed by DNA sequencing, the remaining oligonucleotides predominantly encoded the Tyr-Gly-Gly-Phe-Leu sequence or close variants thereof.
The two experimental groups received intrathecally 10 μl of a mixture containing 10 μM of thiorphan and 20 μM of kelatorphan (to delay the degradation of the peptide ligand) and 10 μM of dermenkephalin (Tyr-D-Met-Phe-His-Leu-Met-Asp-NH 2 , Sigma, a selective δ agonist ( 14, 34)), in 0.9% NaCl.