Words similar to phagescript

Example sentences for: phagescript

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Figure 2shows the map and sequence of templates and primer sites used in BPCR between a portion of plasmid pBS and Phagescript, a derivative of the ssDNA M13 phage.
The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.
Phagescript phage particles, rather than purified DNA prepared from the particles by phenol extraction, were used as the reference template [ 10 ] . The BPCR primer pair was b1 and M13 pI (see figure 2for primer locations) which would give a 1261 bp recombination product.
In the other sample both the pBS (-) (intact template) and the Phagescript DNA (reference template) were intact.
Two samples with known quantities of templates were prepared: one sample contained the pBS (-) sequence template with a cut in the homologous region created by Pvu II digestion (cut template) and the intact ssDNA Phagescript DNA (reference template).