Example sentences for: permeabilized

How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Binding of β-glucuronidase was assayed as described previously [ 31 32 ] . Briefly, cells were permeabilized with 0.25% saponin in 50 mM Hepes (pH 7.0), 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, and 10 mM mannose-6-phosphate (M6P) for 30 minutes on ice.

  • Aliquots of 100 μl (approximately 50,000 cells) from each experimental group were subjected to a cytospin and fixed and permeabilized similarly to the shell vial assay.

  • Third, permeabilization of the cores was monitored by treating an aliquot of the permeabilized cores with micrococcal nuclease and then measuring DNA synthesis by the endogenous polymerase assay.

  • Rat neonatal microglia on glass coverslips were fixed and permeabilized in -20°C methanol for 10 min, and then blocked with 10% goat serum in phosphate buffered saline (PBS) at 25°C for 5 min.

  • Permeabilized cell assays were performed in flow chambers as previously described [ 22 ] . Swiss3T3 cells were flowed into the chamber, the chamber inverted, and cells allowed to spread for 30-45 minutes in a humidified environment at 37°C, 5% CO 2 . The assay was performed by adding 50 ul of the warmed sample solution to the chamber while wicking with Whatman paper.


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