Words similar to permeabilized
Example sentences for: permeabilized
How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:
For mock micrococcal nuclease treatment, the CaCl 2 micrococcal nuclease and EGTA were mixed prior to addition to the permeabilized cores.
If added prior to permeabilization but not during, exogenous actin was still able to incorporate into permeabilized cells and fluorescence incorporation was high, similar to control cells [Figure 1].
For analysis of IL-6 production, the cells were fixed in Cytofix (Pharmingen) for 30 min on ice, then washed and permeabilized with Cytoperm (Pharmingen) for 15 min on ice.
Cells were rinsed with PBS, treated with compounds or media for 1 min, permeabilized in the presence or absence of compound for 3 min with permeabilization buffer (0.
Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).