Words similar to permeabilized
Example sentences for: permeabilized
How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:
Binding of β-glucuronidase was assayed as described previously [ 31 32 ] . Briefly, cells were permeabilized with 0.25% saponin in 50 mM Hepes (pH 7.0), 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, and 10 mM mannose-6-phosphate (M6P) for 30 minutes on ice.
Aliquots of 100 μl (approximately 50,000 cells) from each experimental group were subjected to a cytospin and fixed and permeabilized similarly to the shell vial assay.
Fixed discs were then permeabilized in PBS containing 0.1% saponin and 1% goat serum and incubated overnight with an anti-E2F serum [ 12 ] at 1/250.
To exclude the possibility that the DHBV RNAseH simply could not recognize the specific heteroduplex formed by DRF+ RNA and oligonucleotide D2507-, a second pair oligonucleotides (D2453+ and D2543M-Sal) were employed with DRF+ RNA in oligonucleotide-directed RNAseH assays employing permeabilized DHBV core particles.
However, DNA synthesis was ablated by micrococcal nuclease treatment of permeabilized cores, indicating that permeabilization was successful (data not shown).
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