Example sentences for: permeabilized

How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Mouse osteoblasts were grown on glass coverslips in 24-well plates until they were confluent; they were then fixed and permeabilized using CytoFix/CytoPerm according to the methods recommended by the manufacturer (PharMingen, San Diego, Calif.).

  • Cells were permeabilized with 0.1% Triton X-100/TBS for 3 min, washed twice with TBS, blocked twice for 3 min with 0.1% bovine serum albumin in TBS, and incubated with rhodamine-conjugated phalloidin for 20 min.

  • Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).

  • The cells were fixed and permeabilized with pre-made solutions from Caltag Laboratories (Burlingame, CA) (Fix & Perm) according to kit instructions.

  • Rat neonatal microglia on glass coverslips were fixed and permeabilized in -20°C methanol for 10 min, and then blocked with 10% goat serum in phosphate buffered saline (PBS) at 25°C for 5 min.


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