Words similar to permeabilized

Example sentences for: permeabilized

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Control experiments demonstrated that (i) the cores were permeabilized because the viral nucleic acids were sensitive to micrococcal nuclease following low pH treatment; (ii) the RNAseH substrate could access the polymerase within the viral cores as determined by the trans reaction (Fig.
None of these conditions inactivated the DNA polymerase activity, yet we were unable to detect RNAseH activity in cores permeabilized by any of these methods.
Fixed discs were then permeabilized in PBS containing 0.1% saponin and 1% goat serum and incubated overnight with an anti-E2F serum [ 12 ] at 1/250.
To determine if a similar effect would be observed with the RNAseH activity, an RNAseH assay was performed in permeabilized cores in which the nucleic acids had been removed with micrococcal nuclease.
For analysis of IL-6 production, the cells were fixed in Cytofix (Pharmingen) for 30 min on ice, then washed and permeabilized with Cytoperm (Pharmingen) for 15 min on ice.