Example sentences for: permeabilized

How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were fixed and permeabilized with pre-made solutions from Caltag Laboratories (Burlingame, CA) (Fix & Perm) according to kit instructions.

  • However, DNA synthesis was ablated by micrococcal nuclease treatment of permeabilized cores, indicating that permeabilization was successful (data not shown).

  • Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.

  • Rat neonatal microglia on glass coverslips were fixed and permeabilized in -20°C methanol for 10 min, and then blocked with 10% goat serum in phosphate buffered saline (PBS) at 25°C for 5 min.

  • RNAseH activity in permeabilized cores was measured by oligonucleotide-directed cleavage of internally 32P-labeled RNA substrates.


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