Example sentences for: permeabilized

How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were then washed, permeabilized with 0.1% Triton X-100/ 0.1% Na-Citrate (pH7.

  • At periodic time points, a sample was taken and leukocytes were purified by dextran sedimentation, fixed, permeabilized, and then stained with either anti-TTP or control antibody.

  • We then varied the pH from 7.0 to 8.5; E. coli RNAseH was active at all pHs, but no cleavage was found in reactions containing the permeabilized cores (data not shown).

  • For mock micrococcal nuclease treatment, the CaCl 2 micrococcal nuclease and EGTA were mixed prior to addition to the permeabilized cores.

  • Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.


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