Words similar to permeabilized

Example sentences for: permeabilized

How can you use “permeabilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were fixed for 15 min in 3% (w/v) paraformaldehyde, rinsed for 5 min with 0.05 M NH 4 Cl 2 , permeabilized for 2 min with 0.1% (v/v) TritonX-100, and blocked for 30 min with 0.1% (w/v) BSA.
Oligonucleotide-directed RNAseH assays were performed with permeabilized viral cores under a wide variety of conditions.
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
Nucleic acids within permeabilized cores were digested with micrococcal nuclease in the presence of CaCl 2 Micrococcal nuclease was inactivated by addition of EGTA, 1.5 μg RNA was added, and the reaction was performed under conditions described for the endogenous polymerase reaction.
Third, permeabilization of the cores was monitored by treating an aliquot of the permeabilized cores with micrococcal nuclease and then measuring DNA synthesis by the endogenous polymerase assay.