Example sentences for: permeabilization

How can you use “permeabilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • However, DNA synthesis was ablated by micrococcal nuclease treatment of permeabilized cores, indicating that permeabilization was successful (data not shown).

  • During the permeabilization step, FITC-labelled antibody (p52) was added at a 1:5 dilution.

  • As cytochalasin D has a high affinity (50 nM) for the growing ends of actin filaments, this treatment should inhibit polymerization of existing filaments but should not block new filament formation [ 23 ] . Thus the actin incorporated most likely reflects nucleation of new filaments, as previously discussed [ 24 ] . As expected, cells treated with cytochalasin D during permeabilization showed no actin incorporation (data not shown).

  • Cells that remained flat and were not disrupted by the permeabilization process were counted, and more than 75% (3 trials) showed decreased fluorescence similar to that seen in Figure 1B(cells that showed significant perturbation by the permeabilization process did not show greater actin incorporation).

  • All other immunocytochemical analyses were performed after fixation in 4% paraformaldehyde and 0.15% picric acid, followed by permeabilization in 0.3% Triton X100.


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