Words similar to permeabilization
Example sentences for: permeabilization
How can you use “permeabilization” in a sentence? Here are some example sentences to help you improve your vocabulary:
To detect intracellular TTP, fixed cells were washed twice in permeabilization buffer (consisting of staining buffer with the addition of 1 μg/ml saponin), centrifuged as before, and incubated for 30 min at 4°C in blocking buffer, consisting of 3% IgG with 3% nonfat powdered milk for leukocytes in permeabilization buffer.
Cells were fixed in a permeabilization/fixation buffer (100 mM K-Pipes pH 6.8, 10 mM EGTA, 1 mM MgCL2, 0.2% TritonX-100, 3.7% formaldehyde) for 10 minutes.
All values are expressed as percent LDH released relative to the value obtained following permeabilization of the cells with 50 μM digitonin.
If added prior to permeabilization but not during, exogenous actin was still able to incorporate into permeabilized cells and fluorescence incorporation was high, similar to control cells [Figure 1].
All ethidium bromide fluorescence values were corrected for background (extracellular) dye fluorescence and expressed as a percentage relative to the value obtained following complete permeabilization of the cells with 50 μM digitonin.
Loading...