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Example sentences for: perkinelmer

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The blot was washed once for 5 minutes in PBS-T, incubated with goat-anti-rabbit conjugated HRP (1:10000 dilution) for 1 hour, washed for 5 minutes in PBS-T and developed with Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc.
30–2001) supplemented with 10% fetal calf serum, 10 units/ml penicillin, and 10 μg/ml streptomycin) at 37 °C and 5% CO 2 . For viability studies, cells were seeded in complete growth medium in black 96-well clear bottom ViewPlates (PerkinElmer, Wellesley, Massachusetts, United States) at a density of 5,000 (H1975 and H2030) or 7,500 cells per well (H3255).
The samples were vortexed and were incubated at 100°C for 30 minutes, and the absorbance at 532 nm was measured using a PerkinElmer Lamba 3B spectrophotometer (PerkinElmer, Wellesley, MA, USA).
Calcein fluorescence (Ex, 485 nm; Em, 535 nM) was detected immediately using a Victor V multi-label plate reader (PerkinElmer).
Orientation of the cloned DNA and the integrity of the DNA sequences were confirmed by sequencing with the ABI Prism Dye Terminator Cycle Sequencing kit (PerkinElmer, Wellesley, MA, USA), and searched against the published sequence (TTP accession no.