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Example sentences for: perkin

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The sources of materials used in this study: Phagescript and pBS(-) plasmid were from Stratagene; Taq polymerase and the Stoffel fragment of Taq were from Perkin Elmer Cetus; gp32 was from Pharmacia; Bst E II-digested Lambda DNA was from New England Biolabs.
The synthetic androgen mibolerone (7α-17α-dimethyl-19-nortestosterone) was purchased from Perkin Elmer Life Sciences.
These reactions were carried out in 50 μ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s; annealing at 65°C for 45 s; and extension at 72°C for 40 s.
The RT mix included 5.6 μl of 5X RT buffer, 0.02 mM of dNTPs (with dTTP, not dUTP, Perkin Elmer, Foster City, CA), 14.
PCRs were set up using a laboratory workstation (Biomek 1000; Beckman, Palo Alto, CA) and performed in PEC 9600 thermal cyclers (Perkin Elmer Cetus, Foster City, CA).