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Example sentences for: perkin

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The reactions were set up in 96-well MicroAmp Optical plates (Perkin Elmer) in a 10 μl reaction volume containing 1 U Ampligase (Epicentre Technologies), 20 mM Tris-HCl (pH 8.3), 25 mM KCl, 10 mM MgCl 2 , 0.5 mM NAD, and 0.01% Triton ®X-100.
5 U; Perkin Elmer) with 2'-deoxynucleoside-3'-triphosphates (1.
PCR was performed with the use of Gene Amp PCR Systems 9700 (Perkin Elmer, Watsonville, CA, USA).
Radioactive products were made by 30 cycles of PCR amplification of 10-μl reaction volumes containing 10-30 ng of genomic DNA, 40 ng of each primer, 200 μM each of dATP, dTTP, dGTP, and dCTP, 1 μCi α- 32P-dCTP (3,000 Ci mmol -1) and 0.5 U Taq polymerase (Perkin Elmer).
PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.