Example sentences for: perkin

How can you use “perkin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.

  • Thirty cycles were performed with a Perkin Elmer 9600 thermocycler as follows: 1 min at 95°, 1 min at 55°, 1 min at 72°.

  • PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.

  • Two-step real-time reverse transcription PCR was used to determine the relative expression of mRNA using the ABI Perkin Elmer Prism 5700 Sequence Detection System (Applied Biosystems, Foster City, California, United States) as described previously [38].

  • The PCR reagents were obtained from Perkin Elmer.


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