Words similar to perkin
Example sentences for: perkin
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The PCR primer and the TaqMan fluorogenic probe were designed using the Primer Express program v 1.01 (Perkin Elmer Applied Biosystem, CA, USA).
PCR was performed with the use of Gene Amp PCR Systems 9700 (Perkin Elmer, Watsonville, CA, USA).
For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.
PCR products were then cloned into TA vector (Invitrogen, San Diego, CA, USA), processed using Wizard minipreps (Promega), and sequenced using M13 forward and reverse primers, a DNA Sequencing Kit (Perkin Elmer) and an automated sequenator (Applied Biosystems, Foster City, CA, USA).
PCR reactions were performed using r T th DNA Polymerase (Perkin Elmer), with a 95°C denaturation step for 4 min, and 35 cycles at 58°C for 1 min, 72°C for 3 min, and 94°C for 1 min, and final extension at 72°C for 10 min.
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