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Example sentences for: perkin-

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Confirmation by Taqman (Perkin-Elmer) quantitative PCR was performed for nine selected genes as described in [ 12].
Electroporation of DNA into Y. pestis was done as described previously [ 32 ] . Gene amplification was performed with DeepVent (New England Biolabs, Beverly, MA) or Taq (Eppendorf Scientific, Westbury, NY) DNA polymerase in a Perkin-Elmer GeneAmp Model 2400 thermocycler (Applied Biosystems, Foster City, CA).
PCR reactions (10 mM Tris.HCl, pH 8.3, 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each deoxyribonucleoside triphosphate, each primer at 2 μM, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus) involved 35 cycles of denaturation (95°C for 1.0 min), primer annealing (55°C for 1.0 min), and primer extension (72°C for 2 min).
Purified PCR products were sequenced using an ABI PRISM 377 (Perkin-Elmer, Norwalk, CT) through the Oregon State University Center for Gene Research and Biotechnology.
To amplify the adenoviral DNA, specific primers (Ad5Clip-F: 5'CGACGGATGTGGCAAAAGT3' and Ad5Clip-R: 5'CCTAAAACCGCGCGAAAA3') were designed by using the Primer Express Software (Perkin-Elmer, Foster City, CA, USA).