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Example sentences for: perkin-

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In general, PCR reactions were performed in a volume of 50 μl with 50 picomoles of each primer, dNTP's (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) at a final concentration of 0.5 mM, 1 μl of template, 1X PCR buffer (10X PCR buffer contained 700 mM Tris pH 8.8, 20 mM MgCl 2 , 1% Triton X-100, and 0.1% Tween 20) and Taq polymerase (Perkin-Elmer Inc., Wellesley MA) diluted to the manufacturers recommendations.
DNA sequencing was carried out by cycle sequencing using the PRISM Big Dye Terminator sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Division of PE/ABI).
The structure of all reporter constructs were confirmed by DNA sequencing (Amplitaq sequencing kit, Perkin-Elmer).
Chromatographic analysis of CM-fractions was done by HPLC (Perkin-Elmer Series 410 pump) which was equipped with a LC-235 diode array detector.
One microliter of the diluted product was then added to a mixture containing 12 μl deionized formamide and 0.5 μl molecular weight standard GS 350 TAMRA.The vials were denatured at 95°C/5 min, cooled on ice, and resolved using a 310 Genetic Analyzer (Perkin-Elmer).