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Example sentences for: perkin-

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3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).
Purified PCR products were sequenced using an ABI PRISM 377 (Perkin-Elmer, Norwalk, CT) through the Oregon State University Center for Gene Research and Biotechnology.
To amplify the adenoviral DNA, specific primers (Ad5Clip-F: 5'CGACGGATGTGGCAAAAGT3' and Ad5Clip-R: 5'CCTAAAACCGCGCGAAAA3') were designed by using the Primer Express Software (Perkin-Elmer, Foster City, CA, USA).
PCR (32 cycles) was performed on an ABI 877 Catalyst robotic workstation (5 μl reactions - 5 ng of genomic DNA, 2.5 mM MgCl 2 , 0.2 mM dNTPs [Pharmacia, Piscataway, NJ, USA], 0.2 U Amplitaq Gold DNA polymerase [Perkin-Elmer, Foster City, CA, USA] in 1 × PCR Buffer II [Perkin-Elmer]).
DNA sequencing was carried out by cycle sequencing using the PRISM Big Dye Terminator sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Division of PE/ABI).