Example sentences for: pei

How can you use “pei” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A maximum of ∼ 9% β-gal +neurons were observed at 3 d post-transfection with 1: 5 DNA: PEI, followed by a progressive decrease from 3 d to 10 d.

  • Peak yield was observed at a ratio of 1 μg plasmid DNA to 5 μg PEI (concentration of PEI stock is 1 μg/μl) in 1 ml culture media, producing ∼ 9% transfected cells.

  • Over 30 cell lines have been successfully transfected using PEI, including COS-7 cells [ 8], rat hepatocytes [ 3], human dendritic cells [ 9, 10], and mouse mammary epithelial cells [ 11].

  • As the number of protonatable nitrogens on the linear 22 kD ExGen 500 PEI polymer at physiological pH is roughly equal to 5.47 nmol per μg PEI, and 1 μg DNA corresponds to 3 nmol phosphate groups, this means that the peak yield observed was at a PEI nitrogen: DNA phosphate (N/P) ratio of ∼ 9. Thus, the most suitable N/P ratio for in vitro sympathetic neurons appears to be around 9, creating positively charged DNA/PEI complexes [ 17].

  • Various concentrations of plasmid and PEI (ExGen 500, MBI Fermentas, Hanover, MD) were prepared, using 150 mM NaCl in sterile water as the diluent.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast