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Example sentences for: pei
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Consistent with previous results [ 4, 8, 9, 15, 16,], we found that the β-gal transfection efficiency varied according to the ratio of DNA to PEI (Figure 3).
PEI may be used in conjunction with adenovirus vectors [ 2, 9, 11, 18, 19] and can be conjugated to various ligands for targeted gene delivery [ 10, 16] or to minimize nonspecific interactions with blood when injected into the circulatory system [ 20].
As the number of protonatable nitrogens on the linear 22 kD ExGen 500 PEI polymer at physiological pH is roughly equal to 5.47 nmol per μg PEI, and 1 μg DNA corresponds to 3 nmol phosphate groups, this means that the peak yield observed was at a PEI nitrogen: DNA phosphate (N/P) ratio of ∼ 9. Thus, the most suitable N/P ratio for in vitro sympathetic neurons appears to be around 9, creating positively charged DNA/PEI complexes [ 17].
This finding underscores the versatility of PEI as a method for introducing genes into postmitotic populations of cells, particularly as our previous efforts to transfect sympathetic neurons using lipid-based reagents (Lipofectamine and Lipofectin) had been unsuccessful.
Pei Bank of China Tower, not beloved by the people of Hong Kong — its triangular prisms and sharp angles violate the principles of feng shui (see box, page 68) and its radio masts stick up like an insect’s antennae.
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