Words similar to pei
Example sentences for: pei
How can you use “pei” in a sentence? Here are some example sentences to help you improve your vocabulary:
A maximum of ∼ 9% β-gal +neurons were observed at 3 d post-transfection with 1: 5 DNA: PEI, followed by a progressive decrease from 3 d to 10 d.
Peak yield was observed at a ratio of 1 μg plasmid DNA to 5 μg PEI (concentration of PEI stock is 1 μg/μl) in 1 ml culture media, producing ∼ 9% transfected cells.
Over 30 cell lines have been successfully transfected using PEI, including COS-7 cells [ 8], rat hepatocytes [ 3], human dendritic cells [ 9, 10], and mouse mammary epithelial cells [ 11].
As the number of protonatable nitrogens on the linear 22 kD ExGen 500 PEI polymer at physiological pH is roughly equal to 5.47 nmol per μg PEI, and 1 μg DNA corresponds to 3 nmol phosphate groups, this means that the peak yield observed was at a PEI nitrogen: DNA phosphate (N/P) ratio of ∼ 9. Thus, the most suitable N/P ratio for in vitro sympathetic neurons appears to be around 9, creating positively charged DNA/PEI complexes [ 17].
Various concentrations of plasmid and PEI (ExGen 500, MBI Fermentas, Hanover, MD) were prepared, using 150 mM NaCl in sterile water as the diluent.