Words similar to over-expressing
Example sentences for: over-expressing
How can you use “over-expressing” in a sentence? Here are some example sentences to help you improve your vocabulary:
The genetic event(s) associated with the selection of a virulent variant of the sagA/pel mutant without changing the site or orientation of the Tn 917 transposon was reminiscent of earlier studies from our laboratory testing the virulence of mga mutants of isolate 64/14 [ 9 ] . In that study, injection of an mga mutant, that failed to express any detectable surface M or M-related protein, lead to selection of mga variants over-expressing M and M-related proteins that could be recovered from the spleen following a lethal skin infection.
A partial cDNA clone homologous to AtCOP1 has been previously identified in mammals, containing all three conserved protein-protein interaction domains [ 20 ] . Remarkably, when expressed in plant cells, the mammalian COP1-reporter fusion protein exhibited a light-regulated nuclear localization pattern similar to AtCOP1 [ 20 ] . Although mammalian COP1 failed to rescue the Arabidopsis cop1 mutants, over-expression of the amino-terminal half of mammalian COP1 in Arabidopsis caused a dominant-negative phenotype, resembling the effect of over-expressing the corresponding fragment of AtCOP1 [ 20 21 ] .
Construction of σ E-over-expressing strain of M. smegmatis
The system also provides powerful controls for genetic background effects, because control (no DOX) and over-expressing (+DOX) flies have identical genetic backgrounds and therefore any differences in life span must be due to DOX administration and gene overexpression.
These possibilities could be distinguished by transformation of plasmids from individual clones into the σ E-over-expressing strain and directly comparing of XylE activity in the presence and absence of σ E. Unfortunately, it appears that expression of XylE in M. smegmatis may be detrimental and may lead to plasmid instability and loss of the inserted DNA.
Loading...