Words similar to oligo
Example sentences for: oligo
How can you use “oligo” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).
Competition with 100-fold excess unlabeled CD5X oligo eliminated complexes 1-3 (figure 6a, lane 3).
This also suggests that for both the human-human and littermate-littermate comparisons, no major selective mRNA degradation occurs between tissue harvesting and binding of the mRNA to the magnetic oligo(dT)-coupled beads.
Thus, when the polyA effect is pronounced, the hybridization of the reverse transcription product made from any poly(A) +RNA mixture will be essentially the same, namely, the hybridization that would be obtained with an oligo(dT) probe alone.
Complementary DNA was made using 200 ng of purified, oligo(dT) primed mRNA and SuperScript II RNase H- reverse transcriptase (BRL Life Technologies #18064-014).