Words similar to oligo-d

Example sentences for: oligo-d

How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

mRNA selection from 100 μg total RNA using oligo-d(T) 25 Dynabeads (Dynal) and following the manufacturer's directions yielded 2-3 μg poly(A) +-enriched RNA in 20 μl 10 mM Tris-HCl, pH 7.5.
First and second strand cDNA synthesis was performed using the SuperScript Choice System (Invitrogen AG, Basel, Switzerland), according to manufacturer instructions, but using an oligo-dT primer containing a T7 RNA polymerase binding site.
mRNA was extracted from murine tissues (BALB/c mice: Id wound of controls, 1d wound of mice treated with 0.5 mg Dexamethason per kg bodyweight twice a day for 5 days before wounding) according to standard procedures [ 30 ] . Methylated cDNA was synthesized from an anchored Xho I-oligo-dT primer ((GA) 10 ACTAGTCTCGAGT 16 VN) that secured a defined start of the cDNA synthesis at the cDNA-polyA border using the Stratagene cDNA synthesis kit according to the instructions of the supplier.
Briefly, total RNA (8 μg) from DRGs was reverse transcribed using an oligo-dT primer coupled to a T7 RNA polymerase binding site.
The first and second strand cDNA synthesis was performed using the SuperScript Choice System (Life Technologies) according to manufacturer's instructions, but using oligo-dT primer containing a T7 RNA polymerase binding site.