Words similar to oligo-d
Example sentences for: oligo-d
How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first and second strand cDNA synthesis was performed using the SuperScript Choice System (Life Technologies) according to manufacturer's instructions, but using oligo-dT primer containing a T7 RNA polymerase binding site.
Hybridization probes were prepared according to Affymetrix instruction [ 18 ] . 5 μM primer encoding the T7 RNA polymerase promoter linked to oligo-dT 24 primer was used to prime double-stranded cDNA synthesis from each total RNA sample (25 μg).
Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].
The cDNA synthesis was performed using the cDNA synthesis kit (Supersccript, GBCOL-BRL) using random hexamer and oligo-d(T) primers following the instruction from the manufacturer.
First and second strand cDNA synthesis was performed using the SuperScript Choice System (Invitrogen AG, Basel, Switzerland), according to manufacturer instructions, but using an oligo-dT primer containing a T7 RNA polymerase binding site.