Words similar to oligo-d

Example sentences for: oligo-d

How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

mRNA selection from 100 μg total RNA using oligo-d(T) 25 Dynabeads (Dynal) and following the manufacturer's directions yielded 2-3 μg poly(A) +-enriched RNA in 20 μl 10 mM Tris-HCl, pH 7.5.
Briefly, total RNA (8 μg) from DRGs was reverse transcribed using an oligo-dT primer coupled to a T7 RNA polymerase binding site.
Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].
mRNA was extracted from murine tissues (BALB/c mice: Id wound of controls, 1d wound of mice treated with 0.5 mg Dexamethason per kg bodyweight twice a day for 5 days before wounding) according to standard procedures [ 30 ] . Methylated cDNA was synthesized from an anchored Xho I-oligo-dT primer ((GA) 10 ACTAGTCTCGAGT 16 VN) that secured a defined start of the cDNA synthesis at the cDNA-polyA border using the Stratagene cDNA synthesis kit according to the instructions of the supplier.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).