Example sentences for: oligo-d

How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The first and second strand cDNA synthesis was performed using the SuperScript Choice System (Life Technologies) according to manufacturer's instructions, but using oligo-dT primer containing a T7 RNA polymerase binding site.

  • Hybridization probes were prepared according to Affymetrix instruction [ 18 ] . 5 μM primer encoding the T7 RNA polymerase promoter linked to oligo-dT 24 primer was used to prime double-stranded cDNA synthesis from each total RNA sample (25 μg).

  • Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].

  • The cDNA synthesis was performed using the cDNA synthesis kit (Supersccript, GBCOL-BRL) using random hexamer and oligo-d(T) primers following the instruction from the manufacturer.

  • First and second strand cDNA synthesis was performed using the SuperScript Choice System (Invitrogen AG, Basel, Switzerland), according to manufacturer instructions, but using an oligo-dT primer containing a T7 RNA polymerase binding site.


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