Words similar to oligo-d

Example sentences for: oligo-d

How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

mRNA selection from 100 μg total RNA using oligo-d(T) 25 Dynabeads (Dynal) and following the manufacturer's directions yielded 2-3 μg poly(A) +-enriched RNA in 20 μl 10 mM Tris-HCl, pH 7.5.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
Hybridization probes were prepared according to Affymetrix instruction [ 18 ] . 5 μM primer encoding the T7 RNA polymerase promoter linked to oligo-dT 24 primer was used to prime double-stranded cDNA synthesis from each total RNA sample (25 μg).
Briefly, total RNA (8 μg) from DRGs was reverse transcribed using an oligo-dT primer coupled to a T7 RNA polymerase binding site.
Detailed procedures for isolation of total RNA, enrichment of poly (A)+ RNA by oligo-dT cellulose chromatography and electrophoresis and Northern blotting of RNA have been described previously [ 13 ] . The cDNA probes of mouse RAR α and RAR γ [ 21 22 ] were isolated by agarose electrophoresis after appropriate endonucleases digestion and were radio-labeled with 32P-dCTP, using random priming kit (Boehringer-Mannheim, Indianapolis, IN).