Words similar to oligo-d
Example sentences for: oligo-d
How can you use “oligo-d” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
First and second strand cDNA synthesis was performed using the SuperScript Choice System (Invitrogen AG, Basel, Switzerland), according to manufacturer instructions, but using an oligo-dT primer containing a T7 RNA polymerase binding site.
mRNA was extracted from murine tissues (BALB/c mice: Id wound of controls, 1d wound of mice treated with 0.5 mg Dexamethason per kg bodyweight twice a day for 5 days before wounding) according to standard procedures [ 30 ] . Methylated cDNA was synthesized from an anchored Xho I-oligo-dT primer ((GA) 10 ACTAGTCTCGAGT 16 VN) that secured a defined start of the cDNA synthesis at the cDNA-polyA border using the Stratagene cDNA synthesis kit according to the instructions of the supplier.
mRNA selection from 100 μg total RNA using oligo-d(T) 25 Dynabeads (Dynal) and following the manufacturer's directions yielded 2-3 μg poly(A) +-enriched RNA in 20 μl 10 mM Tris-HCl, pH 7.5.
Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].
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