Words similar to octylglucoside

Example sentences for: octylglucoside

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Cells were labeled with 1 mCi Na 125I and lactoperoxidase-H 2 O 2 , as previously described [ 27 ] . After labeling the cell pellet was washed with ice-cold PBS + NaN 3 , then lysed in 2 volumes of OG Lysis Buffer (200 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , TBS, PMSF) at 4°C.
Delipidated bR in octylglucoside is fully capable of M formation and presumably proton release, although with altered kinetics [ 12 16 ] . The requirement for pR to be in lipid to show fast H +release and M formation stems either from a protein/lipid interaction needed to establish a stable, active tertiary structure, or from the need for the phosphate group in DHPC to act as a proton release group.
Subsequent SDS-PAGE analysis of pR samples that had been stored for periods of time up to several months indicate that after sitting for several weeks in octylglucoside solution at 4°C, the largest post-translational modification on wild-type pR is eliminated - presumably hydrolysed off of the cysteine(s) - leaving only a 31,000-MW band indistinguishable from that seen for pR-TCM (data not shown).
Columns were washed with 10 column volumes of Wash Buffer (40 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , TBS), before elution of bound protein with 10 mM EDTA in Wash Buffer.