Words similar to nucleocapsid
Example sentences for: nucleocapsid
How can you use “nucleocapsid” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RNA genome of RSV is about 15 kb long and contains 11 documented protein-coding genes [ 13 ] . Three viral proteins are minimally required to reconstitute the functional transcription complex of NNR viruses [ 3 ] : the nucleocapsid protein (N) that wraps the negative-strand genome RNA and its full-length complement, the positive-strand antigenome RNA, thus converting them into highly nuclease-resistant, chromatin-like templates; the large protein (L), which is the major subunit of the RdRP; and the phosphoprotein (P), which is the smaller subunit of RdRP and an essential transcription factor of L [ 19 20 21 22 ] . In RSV, optimal transcription, although not replication, additionally requires the transcription antitermination protein M2-1 [ 13 ] . In addition, cellular actin, and to a lesser extent, profilin, are also required for viral transcription [ 17 18 ] .
Interestingly, when this precipitate was probed with a mixture of antibodies against all RSV proteins, in addition to P, the nucleocapsid protein N was detected (lane 7).
Together these proteins transcribe the genomic RNA wrapped with nucleocapsid protein N. It is only the N-RNA complex, and not the naked RNA, that serves as the functional template for the viral RdRP.
We speculate that the genomic and antigenomic RNA of NNR viruses escape the onslaught of dsRNA because they are tightly wrapped with the nucleocapsid protein N, which makes them inaccessible to the dsRNA and/or the RNAi silencing complex (RISC).
The problem is particularly acute for NNR viruses, which have large RNA genomes [ 8 9 ] . The 10-15 kb long RNA genomes of these viruses require specific sequences at the 5' and 3' termini for transcription and replication, and must be properly encapsidated by the nucleocapsid protein (N) in order to be recognized by the viral RdRP [ 15 16 ] . Thus, any recombinant technology must be able to faithfully reproduce these features of the genome.
Loading...