Words similar to nucleation
Example sentences for: nucleation
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Our data in DMSO-water show the same features, which are expected from the complex kinetics of nucleation and growth of insoluble aggregates of UCB diacid (H 2 B), leading to the formation of a new solid phase [ 19 20 ] . Our centrifugation, 5 min at 14,000 g , was quite mild, and the short 20-minute period between preparation of UCB-DMSO-water systems and centrifugation severely limited the time-dependent growth to large aggregates.
Since actin incorporation is not due to elongation of existing filaments and BDM does not affect pure actin dynamics in vitro, we examined the localization of Arp3, a subunit of the actin nucleation Arp2/3 complex.
Both VASP and WAVE function in controlling actin dynamics at the leading edge - VASP is thought to promote filament elongation, and WAVE, actin nucleation [ 25 26 ] . Both VASP and WAVE1 were de-localized from the leading edge after 1 min of BDM treatment [Figure 4].
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
Some variability is expected, however, because of the complexity of the kinetic processes of nucleation, growth and flocculation that precede sedimentation.
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