Words similar to non-specific
Example sentences for: non-specific
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Membranes were washed in Tris-buffered saline containing 0.05% Tween 20 ( TBST ) and non-specific binding sites were blocked by immersing the membrane in blocking reagent (0.
Alexa 488-SP produced the most prominent increase in non-specific fluorescence in the absence of cells.
Given that PIP 2 and PS are negatively charged (with PIP 2 being more negatively charged than PS), a model to account for these binding behaviors is that Boi1's PH domain binds in a non-specific manner to negatively charged surfaces.
Pyridine-2,6-bis(thiocarboxylic acid) (pdtc) is an iron-regulated metabolite produced by certain pseudomonads that is highly reactive towards metals by virtue of a pair of thiocarboxylate groups and ring nitrogen that combine to form a non-specific, high affinity, tri-dentate ligand (Figure 1) [ 1 2 ] . The formation constants for Co, Cu, and Fe complexes of pdtc were determined to be greater than 10 33 [ 3 ] . Uptake studies using 59Fe showed that addition of pdtc to the growth medium of two different pdtc-producing pseudomonads increased the efficiency of iron uptake by 20% [ 1 ] . The contribution of pdtc to iron uptake may be due to the fact that the copper complex of pdtc (Cu:pdtc) has the ability to reduce both soluble and mineral forms of ferric iron) [ 1 ] . Not surprisingly, in light of its high affinity for essential metals, pdtc also exhibits antimicrobial activity [ 4 ] .
Nick-translated biotin-labeled pUC18 plasmid was used as a control for non-specific probe binding and HPV probes were also applied to normal cervical tissues.
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