Words similar to non-specific
Example sentences for: non-specific
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An experiment performed to assess contribution of each of the three sources demonstrated that: (1) non-specific binding of IgG2a-FITC was negligible in all experimental groups (Fig 8H); (2) tumor cells incubated with PMA alone did not bind anti-MHCII mAb above the level of isotype control and (3) tumor cells incubated with PMA (or with PMA+IFNg) demonstrated 1.2-1.
Furthermore, the two allele per well format reduced non-specific amplification.
An early study on DNA microarray hybridization found that hybridization was strongly dependent on the rate constants for DNA adsorption/desorption in the non-probe covered regions of the surface, the two-dimensional diffusion coefficient, and the size of probes and targets and also suggested that sparse probe coverage may provide results equal to or better than those obtained with a surface totally covered with DNA probes [ 19 ] . A theoretical analysis of the kinetics of DNA hybridization demonstrated that diffusion was important in determining the time of required to reach equilibrium and was proportional to the equilibrium binding constant and to the concentration of binding sites [ 20 ] . A recent theoretical and experimental analysis of competitive hybridization in a two-color system demonstrated the need for the hybridization kinetics of the two probes to be the same [ 21 ] . An elegant study on the use of hybridization kinetics to differentiate specific from non-specific binding was recently published [ 22 ] . This study found that the hybridization kinetics for specific and non-specific binding of labeled cRNA to surface-bound oligodeoxyribonucleotides were significantly different, with specific binding requiring longer to reach hybridization equilibrium than non-specific binding.
1A) were initially incubated with an pool of 32P-labeled degenerate oligonucleotides and non-specific competitor DNA poly(dI-dC) (10 μg).
3 years) with non-specific GI symptoms like diarrhea and guaiac positive stool.
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