Words similar to nitrocellulose
Example sentences for: nitrocellulose
How can you use “nitrocellulose” in a sentence? Here are some example sentences to help you improve your vocabulary:
The proteins were then electroblotted to nitrocellulose membranes at 125 mA for 45 min.
An equal mass of each protein sample was subjected to reducing conditions (99°C for 5 min in 100 mM dithiothreitol (DTT)), separated on a 15% SDS-PAGE, and transferred to nitrocellulose membrane according to standard protocols.
Proteins resolved by SDS-PAGE were transferred to nitrocellulose membranes and the membranes blocked for 2 hours in 5% dry milk in PBS-T (Phosphate Buffered saline and 0.1% Tween 20).
The resolved proteins were transferred electrophoretically to nitrocellulose membranes (Hybond-ECL, Amersham Corp.).
When necessary, antibodies were stripped from the nitrocellulose by incubation in stripping buffer [1 M glycine (pH 2.2), 20 mM MgAc, 50 mM KCl] followed by immunoblot blocking solution.
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