Example sentences for: nitrocellulose

How can you use “nitrocellulose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Gels were transferred to nitrocellulose membranes (Sigma) and blocked with 5% milk powder in PBS at 4°C (overnight).

  • The rhabdomyosarcoma cells are cultured and maintained as described [ 37 ] . The cellular proteins were prepared, separated on 4% polyacrylamide gel and transferred onto nitrocellulose membrane.

  • Equal quantities of clarified proteins were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane.

  • The total protein was extracted from approximately 10 7cells by vortexing with glass beads, then resolved by SDS-PAGE on 12% gel, and transferred to nitrocellulose at 55V in (25 mM Tris, 250 mM glycine, 0.1% SDS, and 20% methanol) for 1 hr at room temperature.

  • Proteins were separated on 7.5% Doucet gels [ 48 ] and transferred to nitrocellulose was performed using a wet-transfer apparatus (Bio-Rad) at 100 V for 45 min.


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