Example sentences for: nitrocellulose

How can you use “nitrocellulose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Gels were prepared in duplicate and one of each pair stained in Coomassie blue and the other used for electrophoretic transfer onto a nitrocellulose membrane (Bio-Rad, Hercules, CA).

  • Equal quantities of clarified proteins were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane.

  • Whole cell lysate (50 μg) was loaded onto a 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, transferred to nitrocellulose, and hybridized with an anti-BRCA1 (Ab-1; Oncogene Research, Cambridge, MA, USA) antibody as previously described [ 10].

  • The samples were transferred on to nitrocellulose membranes, which were incubated in blocking buffer (TBS, pH 7.5, containing 10% dried milk and 0.1% Tween-20) and then rabbit anti-Bid antiserum (BD PharMingen, San Diego, CA), 1:2000 dilution in the same blocking buffer, at 4°C, overnight.

  • Proteins were separated on 7.5% Doucet gels [ 48 ] and transferred to nitrocellulose was performed using a wet-transfer apparatus (Bio-Rad) at 100 V for 45 min.


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