Example sentences for: nitroblue

How can you use “nitroblue” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell differentiation was measured by mixing 100 μL aliquots of cells with 100 μL of a solution containing 1.0% w/v bovine serum albumin, 0.2% w/v nitroblue tetrazolium and 1.0 μg/mL of 12-O-tetradecanoylphorbol 13-acetate (TPA).

  • Immunoreactive proteins were detected by alkaline phosphatase reaction with 5-bromo-4-chloro-3-indoyl phosphate/nitroblue tetrazolium.

  • The HL-60 human promyelocytic leukemia cell line responds in culture to sub-micromolar concentrations of retinoic acid ( all-trans and 13-cis ) by undergoing terminal differentiation to a granulocytic cell with the ability to phagocytose and reduce nitroblue tetrazolium [ 28 ] . The response to retinoic acid in HL-60 cells is dependent on the expression of the retinoic acid receptor RAR α [ 29 ] . The use of all-trans retinoic acid in differentiation therapy for patients with acute promyelocytic leukemia has resulted in prolonged, complete remissions when combined with cytotoxic chemotherapy, and may provide complete, long-term remissions as a single agent [ 30 ] .

  • Alkaline phosphatase-conjugated secondary antibody (goat anti-rabbit immunoglobulin G; Pierce) was used to visualize proteins by development with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP; Fisher Scientific, Fair Lawn, NJ).

  • NAD, all-trans retinal, all-trans retinoic acid, dimethylsulfoxide, mammalian protease inhibitor cocktail, nitroblue tetrazolium, and pyrazole were obtained from Sigma Chemical Co., St. Louis, MO.


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