Words similar to nitrocefin

Example sentences for: nitrocefin

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The changes in molar absorptivities (Δε) used to quantitate products were (in M -1cm -1): nitrocefin, Δε 485 = 17,420; cephalothin, Δε 265 = -8,790; cefoxitin, Δε 265 = -7,000; cefaclor, Δε 280 = -6,410; imipenem, Δε 300 = -9,000; meropenem, Δε 293 = -7,600; biapenem, Δε 293 = -8,630; ampicillin, Δε 235 = -809; and penicillin G, Δε 235 = -936.
The results presented here suggests that none of the residues in this study are essential for tight nitrocefin binding, possibly because other parts of the active site accommodate the loss of certain binding contacts.
In reactions with 50 μM mutant and 5 μM nitrocefin, only 0.75 and < 0.30 μM intermediate formed for the Y228F and Y228A mutants, respectively.
It is also possible that the site-directed mutants could be utilizing a different mechanism to hydrolyze nitrocefin [ 66 ] .
However, rapid-scanning Vis studies of N233L and N233D with nitrocefin demonstrate that no detectable amounts of intermediate are formed during the reaction, even when using a wide number of reaction conditions (Figure 2).