Example sentences for: nitrocefin

How can you use “nitrocefin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Stopped-flow fluorescence studies at 10°C with the S224A, S224D, and S224K mutants and nitrocefin as the substrate resulted in K S values of 39 ± 10, 213 ± 63, and 33 ± 5 μM, respectively.

  • When 50 μM S224A was reacted with 5 μM nitrocefin, 1.7 μM intermediate formed during the first 10 milliseconds of the reaction (Figure 2), and rate of decay of this intermediate was equal to the steady-state k cat (Table 3).

  • Nitrocefin was purchased from Becton Dickinson, and solutions of nitrocefin were filtered through a Fisherbrand 0.45 micron syringe filter.

  • When using the cephalosporins, nitrocefin, cefoxitin, and cephalothin, as substrates and the I164A mutant, there were 2- to 10-fold (only for cefoxitin) increases in K m and 2- to 4-fold increases in k cat observed (Table 3).

  • By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.


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