Words similar to nitrocefin

Example sentences for: nitrocefin

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Recently, Spencer and co-workers reported that stopped-flow fluorescence studies can be used to monitor the reaction of L1 with nitrocefin and that an initial binding step can be directly monitored [ 40 ] . By increasing the concentration of nitrocefin, the rate of the initial binding step increased to a maximum, and fitting of these data yielded a binding constant (called K S herein) for nitrocefin.
By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.
Stopped-flow fluorescence studies at 10°C with the N233L and N233D mutants and nitrocefin as substrate resulted in K S values of 26 ± 9 and 25 ± 8 μM, respectively.
To a lesser degree, the aromatic portion of Tyr228 must have an effect on the binding site as the K S value for nitrocefin binding to this mutant is decreased by a factor of 2; however, the hydroxyl group probably does not form a hydrogen bond to the substrate as proposed.
The reaction of wild-type L1 with nitrocefin under steady-state conditions at 10°C resulted in a rapid decrease in fluorescence followed by a rate-limiting return of fluorescence (Figure 3A).