Example sentences for: nitrocefin

How can you use “nitrocefin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Rapid-scanning Vis spectra of nitrocefin hydrolysis by L1 and the L1 mutants were collected on a Applied Photophysics SX.

  • Stopped-flow fluorescence studies at 10°C of nitrocefin hydrolysis by Y191A and Y191F resulted in K S values of 85 ± 9 and 22 ± 6 μM, respectively.

  • In studies with cefoxitin, cefaclor, and cephalothin as substrate, the observed k cat values for the S224A and S224K mutants were 2- to 7-fold lower; however, the k cat values when using nitrocefin as substrate were slightly higher (< 2-fold).

  • To a lesser degree, the aromatic portion of Tyr228 must have an effect on the binding site as the K S value for nitrocefin binding to this mutant is decreased by a factor of 2; however, the hydroxyl group probably does not form a hydrogen bond to the substrate as proposed.

  • We are uncertain why the mutation of residues on the loop that extends over the active site would affect k cat , since substrate and product binding have been predicted to be very fast in the reaction of nitrocefin with L1.


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