Words similar to nitrocefin

Example sentences for: nitrocefin

How can you use “nitrocefin” in a sentence? Here are some example sentences to help you improve your vocabulary:

In studies with cefoxitin, cefaclor, and cephalothin as substrate, the observed k cat values for the S224A and S224K mutants were 2- to 7-fold lower; however, the k cat values when using nitrocefin as substrate were slightly higher (< 2-fold).
Therefore, kinetic studies with nitrocefin as substrate allowed for us to evaluate the effect of point mutations on the reaction mechanism of L1.
To a lesser degree, the aromatic portion of Tyr228 must have an effect on the binding site as the K S value for nitrocefin binding to this mutant is decreased by a factor of 2; however, the hydroxyl group probably does not form a hydrogen bond to the substrate as proposed.
The placement of a negative charge at position 224 drastically affects nitrocefin binding and results in a 6-fold decrease in binding affinity (Table 6).
Steady state kinetic studies were performed on multiple preparations of wild type L1, and the resulting kinetic data are shown in Tables 3, 4, 5. When using nitrocefin as substrate and 50 mM cacodylate, pH 7.0, as buffer, wild-type L1 exhibited a k cat value of 38 ± 1 s -1and a K m value of 12 ± 1 μM.