Words similar to nitrocefin

Example sentences for: nitrocefin

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The reaction of wild-type L1 with nitrocefin under steady-state conditions at 10°C resulted in a rapid decrease in fluorescence followed by a rate-limiting return of fluorescence (Figure 3A).
Rapid-scanning Vis spectra of the reaction of the Y228A and Y228F mutants with nitrocefin demonstrated a marked decrease in the amount of intermediate formed with these mutants (Figure 2).
It is also possible that the site-directed mutants could be utilizing a different mechanism to hydrolyze nitrocefin [ 66 ] .
Absorbance data were converted to concentration data as described previously by McMannus and Crowder [ 39 ] . Stopped-flow fluorescence studies of nitrocefin hydrolysis by L1 were performed on an Applied Photophysics SX.
When using the cephalosporins, nitrocefin, cefoxitin, and cephalothin, as substrates and the I164A mutant, there were 2- to 10-fold (only for cefoxitin) increases in K m and 2- to 4-fold increases in k cat observed (Table 3).