Words similar to nitrocefin

Example sentences for: nitrocefin

How can you use “nitrocefin” in a sentence? Here are some example sentences to help you improve your vocabulary:

In addition, the minimal kinetic mechanism of nitrocefin hydrolysis by L1 has been reported, and this mechanism predicts that K m does not accurately reflect substrate binding.
Steady state kinetic studies were performed on multiple preparations of wild type L1, and the resulting kinetic data are shown in Tables 3, 4, 5. When using nitrocefin as substrate and 50 mM cacodylate, pH 7.0, as buffer, wild-type L1 exhibited a k cat value of 38 ± 1 s -1and a K m value of 12 ± 1 μM.
The placement of a negative charge at position 224 drastically affects nitrocefin binding and results in a 6-fold decrease in binding affinity (Table 6).
Therefore, kinetic studies with nitrocefin as substrate allowed for us to evaluate the effect of point mutations on the reaction mechanism of L1.
The reaction of wild-type L1 with nitrocefin under steady-state conditions at 10°C resulted in a rapid decrease in fluorescence followed by a rate-limiting return of fluorescence (Figure 3A).