Words similar to nitrocefin

Example sentences for: nitrocefin

How can you use “nitrocefin” in a sentence? Here are some example sentences to help you improve your vocabulary:

In reactions with 50 μM mutant and 5 μM nitrocefin, only 0.75 and < 0.30 μM intermediate formed for the Y228F and Y228A mutants, respectively.
To a lesser degree, the aromatic portion of Tyr228 must have an effect on the binding site as the K S value for nitrocefin binding to this mutant is decreased by a factor of 2; however, the hydroxyl group probably does not form a hydrogen bond to the substrate as proposed.
Rapid-scanning Vis studies on nitrocefin hydrolysis by I164A and F158A showed a marked decrease in intermediate accumulation, with the I164A mutant generating < 0.30 μM intermediate and the F158A producing no detectable intermediate (Figure 2).
Stopped-flow fluorescence studies at 10°C with the N233L and N233D mutants and nitrocefin as substrate resulted in K S values of 26 ± 9 and 25 ± 8 μM, respectively.
By using nitrocefin as substrate and K m values alone, a completely different conclusion is reached regarding important substrate binding residues.