Words similar to nitrocefin

Example sentences for: nitrocefin

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The changes in molar absorptivities (Δε) used to quantitate products were (in M -1cm -1): nitrocefin, Δε 485 = 17,420; cephalothin, Δε 265 = -8,790; cefoxitin, Δε 265 = -7,000; cefaclor, Δε 280 = -6,410; imipenem, Δε 300 = -9,000; meropenem, Δε 293 = -7,600; biapenem, Δε 293 = -8,630; ampicillin, Δε 235 = -809; and penicillin G, Δε 235 = -936.
Steady state kinetic studies were performed on multiple preparations of wild type L1, and the resulting kinetic data are shown in Tables 3, 4, 5. When using nitrocefin as substrate and 50 mM cacodylate, pH 7.0, as buffer, wild-type L1 exhibited a k cat value of 38 ± 1 s -1and a K m value of 12 ± 1 μM.
The largest change in K m was observed when cefaclor was used as substrate, and the smallest change was observed when nitrocefin was used as substrate.
Rapid-scanning Vis spectra of the reaction of the Y228A and Y228F mutants with nitrocefin demonstrated a marked decrease in the amount of intermediate formed with these mutants (Figure 2).
By using this mechanism [ 39 ] , K m is equal to {(k -1 + k 2 )k 3 k 4 } / {k 1 (k 3 k 4 + k 2 k 4 + k 2 k 3 }. To probe more directly the reaction, stopped-flow absorbance studies were conducted, and the substrate decay rates (390 nm) were studied as a function of nitrocefin concentration.