Words similar to ni-

Example sentences for: ni-

How can you use “ni-” in a sentence? Here are some example sentences to help you improve your vocabulary:

Recombinant His-tagged P protein was immobilized on a Ni-NTA column and purified recombinant human profilin was passed through it.
Upon affinity purification on Ni-NTA resin, HA-Pop1p/His-Myc-Pop2p complexes were incubated with bacterially expressed, phosphorylated Rum1p.
After centrifugation at maximum speed for 15 minutes, the supernatant was incubated with Ni-NTA resin (Qiagen) for 1.5 hour at room temperature.
The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).
Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .