Words similar to ni-chelation
Example sentences for: ni-chelation
How can you use “ni-chelation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Introduction of the plasmids into E. coli BL21(DE3) cells containing the compatible RIG plasmid [ 39 ] , induction of the proteins with IPTG, and purification through Ni-chelation chromatography were performed using the His-Bind kit reagents and procedure (Novagen).
The recombinant protein was expressed from pET-15b clone and purified by Ni-chelation chromatography (Fig.
The protein was soluble, and was purified by standard Ni-chelation chromatography [ 45 ] . Purified recombinant profilin and antibodies against profilin, RSV P protein, and F protein have been described previously [ 11 25 ] . Polyclonal antibodies to RSV proteins G, N, and M were purchased from Chemicon International (Temecula, CA) and the corresponding rhodamine-conjugated secondary antibody from Sigma.