Words similar to neo
Example sentences for: neo
How can you use “neo” in a sentence? Here are some example sentences to help you improve your vocabulary:
The 3' arm of homology consisted of the 5' end of Neo coding sequence accompanied by the pgk promoter with flanking loxP elements.
Previous Northern blot analysis found high levels of a 2.3 Kb fusion transcript in 1B4 cells [ 29 ] , approximately the size expected for hnRNPA2/neo fusion transcripts terminating in the 5' LTR.
Toward this end, the present study characterised a mutation generated by insertion of the U3Neo gene trap retrovirus into an intron of the hnRNP A2/B1 gene.
It was derived from the plasmid pOS.DUP/DEL that contained the neomycin phosphotransferase (neo) gene that confers resistance to the pharmacological inhibitor G418 and also contains the Herpes Simplex Virus thymidine kinase (HSV-TK) that confers sensitivity to gancyclovir.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).