Words similar to nen
Example sentences for: nen
How can you use “nen” in a sentence? Here are some example sentences to help you improve your vocabulary:
On a glass microarray slide, 2400 know human genes were arrayed into 4 separate grids (A, B, C, D), containing 600 genes each (gene description and location on microarrays available at NEN website: www.nenlifesci.com).
For each gene, stock solutions were prepared containing 1.5 mM MgCl 2 , 1X reaction buffer, 10 μCi of 32P- dCTP (3000 Ci/mmol) (NEN, Boston, MA), 1 μM each primer, and 1.5 U of Taq polymerase (Life Technologies).
Radioligand competition binding assays utilizing CHO cells stably expressing the rSPR were performed with Bolton-Hunter [ 125I] SP (NEN, Boston, MA) at a concentration of 50 pM.
The cells were then washed three times with DMEM without FBS and incubated with 3H-thymidine (1 μCi/ml; NEN, Boston, MA) in DMEM containing 10% FBS for time periods varying between 0 and 30 hours.
After washing, the membranes were incubated with luminol based chemiluminescence reagent (DuPont NEN Research Products, Boston, MA).