Words similar to nco

Example sentences for: nco

How can you use “nco” in a sentence? Here are some example sentences to help you improve your vocabulary:

pJM110 was constructed by cloning a Bam HI- and Nco I-cleaved PCR product of pcrG into pACT2.
pURA4- rec12-Y98F was linearized by digestion with Nco I. Transformation, forward selection for Uracil prototrophy, and reverse selection for FOA resistance were as described [ 61 63 ] . Candidates were screened as described above.
The cDNA probe was prepared by restriction digestion of pET-GST-PPT1 with Bsm I and Nco I. The 879 bp DNA fragment generated, corresponding to 544-1422 of PPT1 coding sequence, was gel-purified and labeled with 0.67 μM [α- 32P] CTP (3000 Ci/mmol) using the DECAprimeII random priming kit (Ambion) according to the user manual.
The resulting deletion reporter plasmids were further modified by mutating the blunted Nco I sites to Bgl II sites.
Plasmids used in this study are described in Table 3. All plasmids expressing truncations of LcrG (pJM1, pJM2, pJM3, pJM4, pJM5, pJM23, pJM52, and pJM53) were constructed by cloning Bam HI- and Nco I-cleaved PCR products into pACT2.