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Example sentences for: nahco
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The neurons (chamber slides) were rinsed twice with fresh low calcium Locke's buffer containing DMSO (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 0.92 mM; glucose, 5.6 mM; HEPES, 5 mM; EGTA, 1 mM; DMSO, 0.625%; pH 7.4) and immersed in the same solution (240 μl per chamber).
At the time of experimental treatment, the culture media was replaced with Locke's buffer containing (in mM) 154 NaCl, 5.6 KCl, 2.3 CaCl 2 , 1 MgCl 2 , 3.6 NaHCO 3 , 5 glucose, 5 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (pH 7.2) and neurons were incubated with culture supernatants from HIV infected monocytic cells, subtoxic concentrations of Tat (40 nM) + gp120 (30 pM), various concentrations of methamphetamine or cocaine, or viral proteins + methamphetamine and cocaine.
4 mM NaHCO 3 , 50 IU heparin sodium) was surgically implanted into the right carotid artery, and another into the left jugular vein.
The neurons (chamber slides) were rinsed twice with fresh low calcium Locke's buffer (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 0.92 mM; glucose, 5.6 mM; HEPES, 5 mM; EGTA, 1 mM; pH 7.4) and immersed in the same solution (240 μl per chamber).
Embryos were enzymatically dechorionated with Pronase and extensively washed in embryo medium prior to injection Embryo medium = 13 mM NaCl; 4.2 mM NaHCO 3 ; 0.54 mM KCl; 0.025 mM Na 2 HPO 4 ; 0.044 mM KH 2 PO 4 ; 1.3 mM CaCl 2 ; 1 mM MgSO 4 ). Embryos were injected and maintained for 14 hours post fertilization on a bed of 0.7% agarose in embryo medium.
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