Words similar to naf
Example sentences for: naf
How can you use “naf” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 M NaCI/1 mM DTT/1 mM PMSF/leupeptin/aprotinin/600 μM Na 3 VO 4 /50 mM NaF).
5 mM, EGTA 0.5 mM, NaF 0.5 mM).
4 , 50 mM NaF, 1 mM sodium orthovanadate, 1 mM AEBSF, 2 ug/ml aprotinin) for 10 minutes on ice.
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].
Cultures were lysed in 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 1 mM DTT, 1% Nonidet P-40, 10% glycerol, 1 mM NaF, 0.1 mM sodium orthovanadate, and protease inhibitors for 30 minutes at 4°C.
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