Words similar to naf
Example sentences for: naf
How can you use “naf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Mutation of Asn122 to Asp by site-directed mutagenesis destroyed the phosphatase activity, confirming the essential role of this residue of PP1 in catalysis [ 19 ] . PfPP1 was inhibited by NaF, inorganic orthophosphate, and pyrophosphate at respective IC 50 values of 2.5 mM, 10 mM, and 90 μM (data not shown).
Smooth muscle preparations were frozen by immersion in ice-cold acetone containing 5 mM NaF (-80°C).
The pipette solution for recording from Xenopus oocytes contained (in mM): 90 NaF, 5 NaCl, 11 EGTA, 10 HEPES at pH 7.4.
Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].