Words similar to naf

Example sentences for: naf

How can you use “naf” in a sentence? Here are some example sentences to help you improve your vocabulary:

Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
Smooth muscle strips were weighed and total protein was extracted by glass-glass homogenization using 50 μl per milligram dry tissue sodium dodecyl sulfate (SDS)-based extraction buffer: 25 mM Tris-HCl, pH 7.4, 2% SDS, 10% glycerol, 1 mM dithiothreitol (DTT), 1 μM leupeptin, 10 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium orthovanadate, 5 mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF).
The pipette solution for recording from Xenopus oocytes contained (in mM): 90 NaF, 5 NaCl, 11 EGTA, 10 HEPES at pH 7.4.
Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].