Example sentences for: nah

How can you use “nah” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For E2F/BrdU double staining, discs were first incubated 30 minutes in a drop of Schneider's medium containing 10% fetal calf serum and 1 mg/ml BrdU then fixed 20 minutes with 2% formaldehyde in PLP (10 mM NaIO 4 , 75 mM Lysine, 37 mM NaH 2 PO 4 , pH 7.2).

  • At the time of analysis, 1.5 ml of the transfected cells were transferred to eppendorf tubes, centrifuged and lysed in 320 μl of lysis buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, and 0.1% Triton X-100 at pH7).

  • Nah!).

  • Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).

  • The resin was washed six times in a denaturing wash buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 6.3) and then boiled for 10 minutes in equal volumes of 2 × SDS loading buffer.


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