Words similar to myosin
Example sentences for: myosin
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In fact, the MHCKs are very likely to be highly regulated enzymes; previous studies have documented the in vitro regulation of MHCK A by autophosphorylation, myosin filaments, and acidic phospholipids [ 32 ] , and data presented here documents that MHCK C can also be regulated via autophosphorylation.
In contrast, loss of cortical and furrow localization is seen for GFP-MHCK-C in the absence of myosin II.
Dictyostelium myosin II was purified as described [ 22 ] . Phosphorylation reactions with myosin II and FLAG-MHCK-C were performed in 20 mM TES pH 7, 2 mM MgCl 2 , and 0.5 mM ATP, with 32P-γ-ATP included at 800 Ci/mol final concentration.
Myosin II heavy chain kinase (MHCK) activity in this system capable of disassembling myosin II filaments in vitro was originally reported with partially enriched kinase fractions [ 13 ] . The enzyme MHCK-A was subsequently purified to homogeneity and shown to be capable of driving myosin II filament disassembly in vitro via myosin II heavy chain phosphorylation [ 14 15 ] ). A MHCK-A cDNA was cloned via expression cloning and peptide sequence derived from the native enzyme [ 16 ] . This enzyme is now recognized as the founding member of a highly novel family of protein kinases unrelated to conventional protein kinases, with members present in D. discoideum and throughout the animal kingdom.
This model is consistent with the recent report that MHCK-A displays enrichment into anterior F-actin-rich protrusions of polarized cells during chemotaxis, and into phagocytic and macropinocytotic extensions [ 23 ] . The polar localization of MHCK-A would be consistent with the long-standing "polar relaxation" model for cytoskeletal reorganization during cytokinesis [ 33 ] . MHCK-A may represent a factor that contributes to polar relaxation in this system via polar disassembly of myosin II filaments.
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